X-Gal
X-Gal: On combination with suitable bacterial cloning vectors, host strains,
| Cat.-no |
Description |
Amount |
Price € |
Shop |
| 406-501 |
X-Gal |
1 g |
49.00 |
add |
| 406-502 |
X-Gal |
5 x 1g |
215.00 |
add |
| 406-503 |
X-Gal |
10 x 1 g |
399.00 |
add |
X-Gal: Order/request by E-mail
X-Gal: Datasheet
X-Gal: Deutsche Beschreibung
Applications:
- blue/white screening
- distinguish between positive and negative clones after transformation
Description:
X-Gal is a highly pure molecular biology grade chemical for colorimetric detection of b-galactosidase activity. It is used for “blue/white screening” of bacterial colonies to differ bacterial or phage clones after transformation. In combination with suitable bacterial cloning vectors, host strains, and IPTG, X-Gal provides an easy way to distinguish between positive and negative clones after transformation.
Quality:
Purity of >98% by HPLC. Tested in the blue/white colony screening experiment.
Note:
- Preparation of a 20 mg/ml stock solution in dimethylformamide or dimethylsulfoxide is recommended.
- Dimethylformamide dissolves some plastic materials. The direct addition of dimethylformamide containing solution to plastic Petri dishes should be avoided.
Storage:
at - 20°C in the dark; shipped at ambient temperature
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