Cat.-no	Description	Amount	Price €	Shop
T500S	Transfection Reagent Maximo	100 µl	FOC *	request a free sample *
T500	Transfection Reagent Maximo	0,5 ml	95.00	add
T510	Transfection Reagent Maximo	2x1 ml	299.00	add

* Only in Germany

Feedback of a customer:
The serum resistant reagent offers a simple procedure: the DNA /transfection reagent complexes can be directly added into complete cell medium and waited for transfection assay.
Without time consuming changing of medium the transfection can be finished in half an hour.

Transfection reagent: Fast protocol
Transfection reagent: Deutsche Beschreibung

Advantages of Maximo Transfection reagent:
- high transfection rate and efficiency
- large gene expression and more transfected cells
- Lower Cytotoxicity

The Maximo-Transfection Reagent has several unique features necessary for efficient transfection - DNA condensation and endosomal release, which improves gene transfection efficiency.

 Based on a cationic polymer Maximo-Transfection Reagent is extremely stable, easy to handle, and more resistant to serum in cell culture.

The above advantages make gene transfection much easier and reproducible. Maximo-Transfection Reagent is widely used for both primary cell and established cell lines.        

 Features and Highlights:

- Compatibility with most cell lines

	The pCMB-luc plasmid was transfected into a number of cell lines. The cells were then assayed for luciferase activity 48 hours post transfection.  Panel (a): Maximo-Transfection Reagent: Panel (b): Competitor A transfection reagent: Panel (C): Competitor B transfection reagent: Panel (d): Competitor C transfection reagent.
- High transfection efficiency  In contrast to other brands Maximo-Transfection Reagent is more stable in presence or in absence of serum, and shows higher transfection efficiency than cationic lipid in some of the most commonly used cell lines. Maximo-Transfection Reagent shows especially higher transfection efficiency in HUVEC (primary cell), which is insensate to most transfection reagents including cationic lipids.	The pCMV-luc plasmid was transfected into HEK 293 cells and then assayed for luciferase activity 48 hours post-transfection.  Panel (a): Maximo-Transfection Reagent; Panel (b): Competitor A transfection reagent: Panel (c): competitor B transfection reagent: Panel (d): Competitor C transfection reagent.
- Low Cytotoxicity The cell survival rate is over 90%, when experiment is carried in suitable condition and with recommended Maximo-Transfection Reagent dosage.	The viability of HEK 293 cells was assessed using MTT method 48 hours post transfection. Each transfection reagent was used under optimal conditions.  Panel (a): Maximo-Transfection Reagent: Panel (b): Competitor A transfection reagent: Panel (c): Competitor B transfection reagent: Panel (d): Competitor C transfection reagent.
	HUVEC cells transfection (100x magnification). Panel (a): Maximo-Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B transfection reagent; Panel (d): Competitor C transfection reagent
	HEK 293 cells were transfected with plasmid DNA containing lacZ gene. Cells incorporating lacZ gene metabolize X-Gal and are stained blue. Cells were assayed 48 hrs post transfection.  Panel (a): Maximo-Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B transfection reagent; Panel (d): Competitor C transfection reagent.
	HEK 293 cells transfected with pEGFPN1 and shown 48 hours post transfection (100x magnification). Panel (a): Maximo-Transfection Reagent; Panel (b): Competitor A transfection reagent; Panel (c): Competitor B transfection reagent; Panel (d): Competitor C transfection reagent.

Protocol:
As a serum resistant reagent, the transfection procedure is very simple: the DNA/transfection reagent complexes can be directly added into complete cell medium and waited for transfection assay With no need to change medium, the transfection can be finished in half an hour, and the whole procedure is simple, short and flexible. Additionally, cytotoxicity is found to be lower with Maximo-Transfection Reagent by carrying out simplified shortened protocol.
 

Cell lines successfully transfected using Maximo-Transfection Reagent (not complete List)

                       

Concentration: 5 mg / ml

Transportation: at room temperature

Storage: + 4°C (do not freeze)

 

>>> Service pages in English <<< >>> Serviceseiten auf Deutsch <<<