Cat.-no	Description	Amount	Price €	Shop
S101	Maximo Taq DNA Polymerase	500 units	49.00	add
S102	Maximo Taq DNA Polymerase	5x500 units	245.00	add
S103	Maximo Taq DNA Polymerase	20x500 units	699.00	add
S104	Maximo Taq DNA Polymerase	100x500 units	on request
S104X	Maximo Taq DNA Polymerase	other amounts	on request

Maximo Taq DNA Polymerase: Order/request by E-mail:
Maximo Taq DNA Polymerase: Datasheet
Maximo Taq DNA Polymerase: Deutsche Beschreibung

Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications:
- Standard / General PCR
- Multiplex PCR
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

Description: 
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: on blue ice

Storage: at -20°C for 24 months

Usage:  

Components	Volume per reaction
10X reaction buffer I or buffer II	5 µl
25 mM MgCl2	1.5 µl (if you use buffer II)
dNTP-Mix (40mM)	1.0 µl
Up-stream primer (10 µM stock)	0,5-2.5 µl
Down-stream primer (10µM stock)	0.5-2,5 µl
Template DNA	0.1-15 ng/ml plasmid DNA  1-10 µg/ml genomic DNA
Maximo Taq DNA Polymerase (5 u/µl)	0.2 - 1.0 µl
Sterile dest. Water (molecular grade)	up to 50 µl total reaction volume

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	2-5 min	94-95°C
25-30 Cycles:  Denaturation  Annealing  Extension	10-25 sec  10-25 sec  60 sec	94-95°C  55-65°C  72°C per 1kb
Final extension	5 min	72°C

 

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