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You are here : PCR / DNA AmplificationPolymerases ╗ Taq DNA Polymerase

Taq DNA Polymerase

Maximo Taq Polymerase is a highly purified polymerase for routine amplification

 
 
Cat.-no Description Amount Price € Shop
 S101   Maximo Taq DNA Polymerase          500 units      39.00  add
 S102   Maximo Taq DNA Polymerase      5x500 units    195.00  add
 S103   Maximo Taq DNA Polymerase    20x500 units    499.00  add
 S104   Maximo Taq DNA Polymerase  100x500 units  on request  
 S104X   Maximo Taq DNA Polymerase  other amounts  on request  

Maximo Taq DNA Polymerase: Order/request by E-mail:

Maximo Taq DNA Polymerase: Datasheet

Maximo Taq DNA Polymerase: Deutsche Beschreibung


Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications:
- Standard / General PCR
- Multiplex PCR
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

Description: 
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:
  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I: 
500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl2

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: 
on blue ice


Storage:
at -20°C for 24 months

Usage:  

Components Volume per reaction
 10X reaction buffer I or buffer II  5 µl
 25 mM MgCl2  1.5 µl (if you use buffer II)
 dNTP-Mix (40mM)  1.0 µl
 Up-stream primer (10 µM stock)  0,5-2.5 µl
 Down-stream primer (10µM stock)   0.5-2,5 µl
 Template DNA  0.1-15 ng/ml plasmid DNA
 1-10 µg/ml genomic DNA
 Maximo Taq DNA Polymerase (5 u/µl)   0.2 - 1.0 µl  
 Sterile dest. Water (molecular grade)  up to 50 µl total reaction volume

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl2 concentration for best result

General Thermo-Cycler protocol:

 Step  Time  Temperature
 Initial denaturation  2-5 min  94-95°C
 
25-30 Cycles:
 Denaturation
 Annealing
 Extension
 
 10-25 sec
 10-25 sec
 60 sec

 94-95°C
 55-65°C
 72°C per 1kb
 
 Final extension    5 min  72°C

Maximo Taq Polymerase und Taq polymerase Blue ready to load














































 

>>> Service pages in English <<< >>> Serviceseiten auf Deutsch <<<
Copyrightę GeneOn 2007-15 print page: Taq DNA Polymerase
 


 
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Polymerases
 
one-Fusion high-speed-fidelity Polymerase
HighEnd and exclusive Polymerase: faster speed, more accuracy, better result
DFS Taq Polymerase DNA-free Discontinued
DFS Taq Polymerase (DNA free) is a highly purified polymerase (DNA-free) especially for PCR reactions where freedom from endogenous template (i.e., E. coli DNA) is essential
DFS-PLUS Taq Polymerase DNA-free
DFS PlusTaq Polymerase: more sensitivity; more processivity
H-SPlus-Taq DNA Polymerase
Hot Start Polymerase with ultra short inactivation time, free of MAB´s. The high concentrated versions are designed for Lyophilization
Hot-Start PCR: M-Superhot Taq Polymerase
Superior Taq Polymerase for Real Time PCR and Hot-Start PCR, low-copy number PCR, PCR of difficult templates, Hot-Start activity (only 5 min initial denaturation). The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. The enzyme provides a convenient setting up at room temperature
SNPase for SNP-Genotyping
Hot-Start Polymerase for SNP detection by allele-specific PCR and micro sequencing
Taq DNA Polymerase
Maximo Taq Polymerase is a highly purified polymerase for routine amplification
Taq DNA Polymerase 2X-preMix
Maximo Tag Polymerase as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR
Taq DNA Polymerase blue ready-to-load
Maximo Tag-Blue Polymerase is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed!
m-Anti-Taq
Enhancer for Polymerase reactions
p-Taq Polymerase
p-Taq Polymerase is developed to enhance the specificity and yield of DNA amplification. The polymerase provides a convenient setting up at room temperature because of blocking the the enzyme with optimized mix of inhibitors
Bst DNA Polymerase
Bst DNA allows sequencing through problematic secondary structures
Pfu/Psp DNA Polymerase
Pfu DNA polymerase generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragments are blunt-ended for direct ligation
Pfu/Psp red DNA Polymerase RTL
Pfu DNA polymerase (RTU=READY-TO-LOAD) is a convenient Mixture of proof-reading Enzyme, red dye and loading buffer. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Pfu/Psp DNA Polymerase 2X-preMix
Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Tth DNA Polymerase
Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium.


 
 
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