SuperHot qPCR Master Mix HY

PCR Master mix for Real time and Hot-Start PCR, optimized for "HIGH YIELD" PCR results

Cat.-no	Description	Amount	Price €	Shop
S250	SuperHot qPCR Master mix HY (2x1,25 ml)	100 rcs / 50µl	69.00	add

qPCR Master Mix HY: Datasheet
qPCR Master Mix HY: Deutsche Beschreibung

Features:
- The Master Mix (2X) is optimized for "high yield" PCR results
- Time saving ready-to-use qPCR Mastermix
- repeatable and reliable results
- efficient PCR for a wide range of template concentrations
- activation time < 3 minutes

Applications:
- Realtime PCR and quantitative PCR e.g. with Sybr green, Eva green or probes
- DNA Labeling with biotin or radioactive nucleotides
- Multiplex PCR
- Sequencing of of double or single stranded DNA
- Low copy targets PCR

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity of the mix is achieved by an optimized hot-start polymerase and an optimized Buffer system. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix HY
Hot-Start Polymerase (m-Superhot-Taq) for qPCR, dATP, dCTP, dGTP, dTTP, reaction buffer with stabilizers and enhancers for "High-Yield" results, 1 Tube PCR-grade water, 1 Tube MgCl₂Quality control:
- Performance and purity tests
- Endodeoxyribonuclease Assay
- Real time PCR Test with SmartCycler II
- PCR Test with Lambda DNA (12 kb-fragment) and human placental DNA (3 kb-fragment)

Transportation: on blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months

Components	Volume per reaction	final conc.
2X qPCR / RTD-PCR Master Mix HS	25 µl	1x
Up-stream primer (10 µM stock)	1,5 µl (range: 0,5-2.5 µl)	300 nM
Down-stream primer (10µM stock)	1,5 µl (range: 0.5-2,5 µl	300 nM
reference dye (optional)	x µl	NA
Template DNA	5 µl (0.1-15 ng/ml plasmid DNA) (1-10 µg/ml genomic DNA)	< 500ng DNA
Sterile dest. Water (included)	up to 50 µl total reaction volume

- vortex all solutions carefully before using and before PCR
- may you add the enzyme mix after Template DNA
- an individual optimization of annealing temperature may be necessary for new combinations of primers
and Template DNA
Note: Do not use DMSO or Formamide

General Thermo-Cycler protocol:

Step Time Temperature

Initial denaturation 1-3 min 95°C

35-50 Cycles:
Denaturation
Annealing
Extension

15-30 sec
30-65 sec
30 sec
(per 500bp)

95°C
55-65°C
72-75°C

Note:
- an individual optimization of annealing temperature may be necessary for new combinations of primers
and Template DNA

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