RsaI

Sequence of RsaI: 5´...GTAC...3´ 3´...CATG...5´

Catalog-no	Description	units	Euro	Order by E-mail	Shop
250-129S	RsaI	1000 u	18.00	RsaI 1000 u	Add
250-129L	RsaI	5x1000 u	90.00	RsaI 5000 u	Add

RsaI: Datasheet
RsaI: Order by E-Mail

Overhang: 5´ - blunt

Cut Site:

G T / A C
C A / T G

Isoschizomers: Afa I

Neoschizomers: Csp6 I

Source: Rhodopseudomonas sphaeroides.

Buffer supplied: 10x M.

Substrate for unit definition: λ DNA.

Reaction conditions: 50 mM NaCl, 10 Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.

Store: at -20°C.

Absence of contaminants: Forty units of RsaI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with RsaI, more than 95% of the DNA fragments can be ligated and recut with this enzyme.

Heat inactivation: 65°C for 20 minutes.

Note: Cleaves single-stranded DNA slowly. Activity may be blocked by some combinations overlapping CpG methylation.

Table of Restriction Endonucleases

Alu I - BseC I BshF I - Hpa I Kpn I - Pvu II Rsa I - SnaB I SseB I - Xba I

Alu I BshF I Kpn I Rsa I SseB I

ApaL I BsiS I (N/A) Mbo I Sal I Sst I

Asu II BssA I MspC I Sau3A I Sty I

BamH I BstE II Nae I Sca I Taq I

Bcl I CspA I Nco I Sfi I Xba I

Bgl I EcoR I Nhe I SgrB I (Sac II)

Bgl II EcoR V Not I Sla I

BseA I Hind III Nru I Sma I

BseB I Hinf I Pst I SnaB I

BseC I Hpa I Pvu II Sph I

Table of Isoschizomeres

Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye

Aat I SseB I BsiX BseC I Cbi I Asu II NgoM IV Nae I

Acc III BseA I BIsp106 I BseC I Cfr42 I SgrB I NspV Asu II

Afi II MspC I Bsp119 I Asu II Cla I BseC I PaeR7 I Sla I

Age I CspA I Bsp13 I BseA I Csp45 I Asu II Pal I BshF I

AsiA II CspA I BspD I BseC I Eco147 I SseB I PinA I CspA I

Ban III BseC I BspE I BseA I EcoR II BseB I Pm55 II SseB I

Bfr I MspCI BspT 1 MspC I Hae III BshF I PspG I BseB I

Bpu14 I Asu II BspX I BseC I Kpn I BseA I Sac II SgrB I

Bsa29 I BseC I Bst2U I BseB I Ksp I SgrB I Sfr274 I Sla I

Bsc I BseC I Bst98 I MspC I Lsp I Asu II Sfr303 I SgrB I

Bsh I BshF I BstB I Asu II Mro I BseA I Sfu I Asu II

Bsh T I CspA I BstN I BseB I MroN I Nae I Sst II SgrB I

BsiC I Asu II BstO I BseB I Msp I BsiS I Stu I SseB I

BsiL I BseB I Bsu15 I BseC I Mva I BseB I Tlil Sla I

BsiM I BseA I BsuR I BshF I NgoA IV Nae I Xho I SseB I

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