RNA Ladder HighRange

RNA Ladders from GeneOn are ideal for sizing and quantification of single-stranded RNA fragments

Cat.-no	Description	Amount	Price €	Shop
300023	RNA Ladder HighRange, 200 - 6000 b	5 x 10 µg	129.00	add
300024	RNA Ladder HighRange, 200 - 6000 b	15 x 10 µg	349.00	add

RNA Ladder HighRange: Order by E-mail
RNA Ladder HighRange: Datasheet
RNA Ladder HighRange: Deutsche Beschreibung

Features:
The ladder is designed for qualitative and quantitative analysis of RNA on agarose gels with ethidium bromide or Sybr-Green II as dyes.

Description:
Mixture off 8 RNA transcripts from specific templates, among others some l-sequences and one part
of the pTZ19R poly linker, supplied in 1x Loading buffer. The RNA-Ladder is suitable for the analysis
of RNA in native or denatured Agarose gels and can be stained by ethidium bromide.The RNA-Ladder is suitable for the analysis of RNA in native or denatured agarose gels (0.8 - 2 %) and can be stained by ethidium bromide.

Concentration: 0.5 mg RNA/ml

2X RNA Tracking dye (included):
95 % formamide, 0.025 % SDS, 0.025 % bromphenol blue, 0.025 % xylene cyanol, 0.025% ethidium bromide and 0.5 mM EDTA

1X Loading buffer (included): 10 mM K-Acetate (pH 4.5), 47.5 % formamide, 0.0125 % SDS, 0.0125 % bromophenol blue, 0.0125 % xylene cyanol, 0.0125 % ethidium bromide and 0.25 mM EDTA

Storage Buffer: 1 mM EDTA (pH 6.0)

Number of bands: 8 6000, 4000, 3000, 2000, 1500, 1000, 500 and 200 bases

Quality control/results:
- Absence of ribunucleases
- Determination of RNA concentration by spectralphotometer
- free of degrated RNA and NTP's

Electrophoresis:
- denaturing formaldehyde agarose with MOPS buffer
- denaturing polyacrylamide gel electrophoresis in TBE buffer
- native 2% agarose with TAE buffer
- denaturing glyoxal/DMSO agarose with sodium phosphate buffer

Loading for native or denaturing agarose- and polyacrylamide gels
HighRange RNA-Ladder has to be mixed with Loading buffer 2X before use (use the same amount of RNA relating to 1 µg of loaded marker)

- Thaw the ladder on ice and mix well by pipetting or gentle vortexing
- For 8 mm lane width prepare:
- 2 μl 2x Loading buffer with 2 μl RNA-Ladder
- Vortex briefly and spin down
- Heat at 70 °C for 10 minutes
- Chill quickly on ice and load the gel
- Use 0.5 μl (0.125 μg) per mm lane width
After finishing gel electrophoresis stain the gel with ethidium bromide.

Use the same volume of RNA samples and marker. Dilute the samples with 2x Loading buffer and DEPC water. Additionally the concentration of loading buffer in
samples and marker should be equal.

Note: RNA is sensitive to degradation by ribonucleases. Working with any RNA Ladder of GeneOn all components have to be prepared fresh. All required tools and consumables should be treated with e.g. diethyl pyrocarbonate. Protect your hands with resistant gloves and your eyes with goggles!

Transportation: Shipped on blue ice

Storage: at -20°C/ -70°C for 12 months

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