Reverse Transcription (RT-PCR)

RT-PCR: MMLV Reverse Transcriptase, AMV Reverse Transcriptase or Tth DNA Polymerase for the synthesis of cDNA

Product	Short description
MMLV Reverse Transcriptase	For reverase transcription, RNase H ^-, for high yield cDNA
AMV Reverse Transcriptase	For reverase transcription, RNase H⁺, for high specifity
Ribonuclease Inhibitor RNase Inhibitor	For inhibition of the activity of different RNases
Tth DNA Polymerase	Thermostable enzyme for reverse transcription
Random Primers	Single-stranded random hexanucleotides mixture
Oligo (dT)₁₅	Standard primer for RT-PCR

Reverse Transkription (RT-PCR) : Deutsche Beschreibung

Products for RT-PCR: Overview

MMLV Reverse Transcriptase

MMLV Reverse Transcriptase (for RT-PCR), encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized

AMV Reverse Transcriptase

AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus reverse transcriptase and expressed in E.coli. is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template

Ribonuclease (RNase) Inhibitor

RNase Inhibitor is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C

Tth DNA Polymerase

Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium.

Random Primers

Mixture of single-stranded random hexanucleotides

Oligo (dT)15

Standard primer for RT-PCR

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