qPCR/RTD PCR Mastermixes SaveLine: DLP for dual labeled DNA fluorescent  Probes *) and PCR cyclers with block techology	Short description
qPCR Master Mix DLP1	For quantitative Real-time PCR. The Mastermix contains  dUTP instead of dTTP. The Mix can be used with ROX  dye as reference in compatible cyclers.
qPCR Master Mix DLP2 with UNG	For quantitative Real-time PCR. The Mastermix contains  dUTP instead of dTTP and UNG. The Mix can be used with ROX dye as reference in compatible cyclers.
qPCR Master Mix DLP3 with ROX	For quantitative Real-time PCR. The Mastermix contains ROX as reference dye and dUTP instead of dTTP.
qPCR Master Mix DLP4 ROX/UNG	For quantitative Real-time PCR. The Mastermix contains ROX as reference dye, dUTP instead of dTTP and UNG.

*) e.g. FRET probes, Molecular Beacons, Taqman

qPCR und RTD Mastermixe: Deutsche Beschreibung


Advantages of qPCR and RTD-PCR Mastermixes from GeneON:

- The Master Mixes contain dUTP. This prevent carry-over contamination of  previous PCR reactions using
  UDG treatment
- The Master Mixes contain a Hot-start enzyme which needs only a short first denaturation step of less
  than 5 minutes
- The Master Mixes are ready-to-use; all components: Hot-start enzyme, dATP, dCTP, dGTP, dUTP, 
   reaction buffer with KCl and MgCl₂, special enhancer and stabilizers are optimized.
- PCR-grade water is provided
- The amount of reactions is calculated for 50µl PCR assays
- Dual Labeled Fluorescent Probes: All common dye-quencher combinations 
-  Reporter: FAM, TET, Cal Fluor Gold 540, JOE, HEX, CAL Fluor Orange 560, Cy3,
   TAMRA, ROX, Cal Fluor Red 610, Cy5, IRD700





qPCR Mastermixe for dual labeled probes: Overview

qPCR/real-time-PCR Master Mix DLP1

qPCR/RTD-PCR Master mix with dUTP

qPCR/real-time PCR Master Mix DLP2

The master mix for RTD-PCR/qPCR contains: dUTP and UDG

qPCR/real-time PCR Master Mix DLP3

The Master mix for RTD-PCR/qPCR contains: dUTP and ROX (500 nM)

qPCR/real-time PCR Master Mix DLP4

The Master mix for RTD-PCR/qPCR contains: dUTP. UNG and ROX (500 nM)

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