pUC19 DNA/BsiS I (Hpa II) digest
DNA Sizer: pUC19 DNA/BsiS I (Hpa II) digest is ideal for qualitative analysis of dsDNA
| Cat.-no | Description | Amount | Price € | Shop |
| 300042 | pUC19 DNA/BsiS I | 2 x 100 µg | 120.00 | add |
| 300043 | pUC19 DNA/BsiS I | 5 x 100 µg | 550.00 | add |
pUC19 DNA/BsiS I (Hpa II): Order E-mail:
pUC19 DNA/BsiS I (Hpa II): Datasheet
pUC19 DNA/BsiS I (Hpa II): Deutsche Beschreibung
Features: 6X Loading buffer required
Description/Preparation:
The BsiS I digest of pUC19 DNA yields 13 discrete fragments (in base pairs): 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26.
Preparation: pUC19 DNA was completely digested by BsiS I, phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Storage buffer: 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA
Usage: 0,5 µg/lane
Concentration: 0.2-0.5 µg/µl
Number of bands: 13 501, 489, 404, 331, 242, 190, 147, 111, 110, 67, 34, 34, 26.
Loading: Agarose Gel / Polyacrylamide Gel
- Vortex gently before using
- apply 0,5 µg (agarose) or 0.5-0.9 µg (polyacrylamide gel) per 1 mm lane
Quantification:
See the graph for the percentage of the bands per band in ng, relating to 0.5 μg loaded marker. Use the same volume of DNA and marker. Additionally the concentration of loading buffer in samples and marker should be equal.
Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
Transportation: Shipped on blue ice or room temperature
Storage: at -20°C for 24 months
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