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You are here : PCR / DNA AmplificationEnzymes and chemicals for PCR ╗ Proteinase K

Proteinase K

Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable

 
 
Cat.-no Description Amount Price € Shop
 405-001  Proteinase K
 20 mg/ml *
    5x1 ml    42.00  add
 405-002-25  Proteinase K  4x25 mg    52.00  add
 405-002  Proteinase K    200 mg    79.00  add 
 405-010  Proteinase K  1000 mg ***  175.00  add

* Storage Buffer:
50 mM Tris, pH 8, 3 mM CaCl2, glycerol 50% (other concentrations of Proteinase K on request)

*** customized amounts available: e.g. 40 mg / vial; 75 mg / vial

Proteinase K: Order/request by E-mail

Proteinase K: Datasheet

Proteinase K: Deutsche Beschreibung

Proteinase K: MSDS


Application:
- Digestion of proteins
 
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).

Activity:  > 30 units/mg protein (hemoglobin, pH 7.5, 37°C)

Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.

Features:
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is
strongly inhibited by PMSF.

Usage:
In presence of 0.5 – 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable

Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature

Information about Proteinase K from Applichem GmbH:
Proteinase K belongs to the familiy of subtilisin-like serine proteases. It has an endo- and exoproteolytic activity. Activated by calcium (1 - 5 mM), the enzyme digests proteins preferentially after hydrophobic amino acids (aliphatic, aromatic and other hydrophobic amino acids).

Proteins will be completely digested, if the incubation time is long and the protease concentration high enough. Upon removal of the calcium ions, the stability of the enzyme is reduced, but the proteolytic activity remains (3). Proteinase K has two binding sites for Ca2+, which are located close to the active center, but are not directly involved in the catalytic mechanism. Removal of the Ca2+-ions reduces the catalytic activity of proteinase K by 80 %.

The residual activity is sufficient to digest proteins, which usually contaminate nucleic acid preparations. Therefore, the digest with proteinase K for the purification of nucleic acids is performed in the presence of EDTA (inhibition of magnesium-dependent enzymes). Is the presence of Ca2+ required, Ca2+ is added up to a concentration of 1 mM and is removed by the addition of EGTA (pH 8.0; final conc. 2 mM) later on.

The pH-optimum is at 8, but the enzyme is active over a wide pH-range (pH 4.3 - 12). An elevation of the reaction temperature from 37°C to 50 - 60°C may increase the activity several times, like the addition of 0.5 - 1 % SDS. Temperatures above 65°C, trichloroacetic acid or the serine protease-inhibitors AEBSF, PMSF or DFP inhibit the activity. Proteinase K will not be inhibited by EDTA (see ref. 2), urea (1 - 4 M), SDS, citrate, iodoacetic acid or, interestingly, by other serine protease inhibitors like TLCK and TPCK. In case that proteinase K has to be inactivated, make sure, that the temperature is not below 95°C and the time not shorter than 10 minutes. A TCA-precipitation is well suited too.

Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases. Some examples for applications: Purification of genomic DNA from bacteria (miniprep): Bacteria from a saturated liquid culture are lysed and proteins are removed by a digest with 100 μg/ml proteinase K for 1 h at 37°C (ref. 1 Suppl. 40 page 2.4.1);

Whole-Mount in situ hybridization and determination of RNAs in vertebrate embryos and isolated organs: Digest of the sample with e. g. 10 μg/ml proteinase K for 15 minutes at room temperature; The period of the treatment and/or the concentration of teh enzyme has to be optimized (ref. 1 Suppl. 35 page 14.9.3); Prepration of DNA from cells or tissue for PCR: Cells or tissue are incubated over night at 50°C with 100 μg/ml proteinase K (ref. 1 Suppl. 17 page 15.3.1); Isolation of vaccinia virus DNA: Digest the virus in a suspension with 2 mg/ml proteinase K for 4 h at 37°C (ref. 1 Suppl. 43 page 16.17.8); Before the phenol extraction for the purification of nucleic acids is performed, a digest with proteinase K may be introduced (50 - 200 μg/ml final concentration; 37°C for 30 minutes in the presence of SDS; ref. 4). We recommend a working concentration between 10 - 100 μg/ml.

Stabiliy: Lyophilized proteinase K is stable at +4°C for at least 12 months. In solution, the stabiliy is approx. 6 - 12 months at +4°C to -20°C .

Stock solutions (10 - 20 mg/ml) may be prepared in 10 mM CaCl2 or 50 mM Tris · HCl, pH 8.0; 1 mM CaCl2 or 50 % Glycerol; 20 mM Tris · HCl, pH 7.4; 1 mM CaCl2 or 50 % Glycerol; 50 mM Tris · HCl, pH 8.0; 1 mM CaCl2

References:
  1. Betzel, C., Three Dimensional Structure of Proteinase K at 0.15 nm Resolution. Eur. J. Biochem., 178, 155-171 (1988).
  2. Ebeling, W., et al., Proteinase K from Tritirachium album Linder, Eur. J. Biochem., 47, 91 (1974).
  3. Enzymes of Molecular Biology, vol. 16, Burrell, M.M., ed. Humana Press (Totowa, NJ: 1993), p. 307. Kraus, E., and Femfert, U., Proteinase K from the Mold Tritirachium album Limber, Specificity and Mode of Action. Z. Physiol. Chem., 357, 937 (1976).
  4. Lizardi, P.M., and Engelberg, A., Rapid Isolation of RNA Using Proteinase K and Sodium Perchlorate. Anal. Biochem., 98, 116 (1979).
  5. Gross-Bellard, et al., Isolation of High Molecular Weight DNA from Mammalian Cells, Eur. J. Biochem., 36, 32-38 (1973).
  6. Molecular Cloning: A Laboratory Handbook, 2nd ed., Sambrook et al., eds., Cold Spring Harbor Press (Cold Spring Harbor, NY: 1989) p. 1.61 and p. B.16.
  7. Kasche, V., et al., A Two-step Procedure for Quantitative Isolation of Pure Double-strand DNA from Animal Tissues and Cell Cultures. Prep. Biochem., 11, 233 (1981).
  8. Hansen, J.N., Isolation of Higher Molecular Weight DNA from Bacillus cereus T Using Proteinase K. Prep. Biochem., 4, 473 (1974).
  9. Holm, C., et al., A Rapid, Efficient Method for Isolating DNA from Yeast. Gene, 42, 169 (1986).
  10. La Claire, J.W., and Herrin, D.L., Co-isolation of High-Quality DNA and RNA from Coenocytic Green Algae. Plant Mol. Biol. Reporter, 15, 263 (1997).
  11. Petsch, P., et al., Proteinase K Digestion of Proteins Improves Detection of Bacterial Endotoxins by the Limulus Amebocyte Assay: Application for Endotoxin removal from Cationic Proteins. Anal. Biochem., 259, 42 (1998).
  12. Brdiczyka, D., and Krebs, W., Localization of Enzymes by Means of Proteases. Biochem. Biophys. Acta, 297, 203 (1973).
  13. Short, B.G., et al., Automated Double Labeling of Proliferation and Apoptosis in Glutathione S-transferase-positive Hepatocytes in Rats. J. Histochemistry and Cytochemistry, 45, 1299 (1997).
  14. Angerer, L.M., et al., Identification of Tissue-Specific Gene Expression by in-situ Hybridization. Methods in Enzymology, 152, 649 (1987).
  15. Sakaguchi, S., et al., Accumulation of Proteinase K-Resistant Prion Protein (PrP) is Restricted by the Expression Level of Normal PrP in Mice Inoculated with a Mouse-Adapted strain of the Creutzfeldt-Jakob Disease Agent. J. Virology, 69, 7586 (1995).
  16. Bennion, B.J., and Daggett, V., Protein Conformation and Diagnostic Tests: the Prion Protein. Clinical Chemistry, 48, 2105 (2002).
  17. Hori, R., and Carey, M., Protease Footprinting Analysis of Ternary Complex Formation by Human TFIIA. J. Biol. Chem., 272, 1180 (1997).
  18. Hilz, H., et al., Stimulation of Proteinase K action by Denaturing Agents: Application to the Isolation of Nucleic Acids and the degradation of “Masked” Proteins. Eur. J. Biochem., 56, 103 (1975).
  19. Methods of Enzymatic Analysis, 3rd Edition, Bergmeyer, H.U., ed., Academic Press (New York, NY: 1983) vol. 2, p. 299.
  20. Jany, K.D., et al., Amino Acid Sequence of Proteinase K from the Mold, Tritirachium album Linder. Proteinase K; a Subtilisin-related Enzyme with Disulfide Bonds. FEBS Letters, 199, 139 (1986).
  21. Jany, K.D., and Mayer, B., Proteinase K from Tritirachium album linder, Molecular Mass and Sequence Around the Active Serine Residue. Biol. Chem. Hoppe-Seyler, 366, 485 (1985).
  22. Bajorath, J., et al., The Enzymatic Efficiency of Proteinase K is Controlled by Calcium. Eur. J. Biochem., 176, 441-447 (1988).
  23. IUBMB Enzyme Nomenclature: http://www.chem.qmul.ac.uk/iubmb/enzyme/EC3/4/21/64.html
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Overview enzymes
and chemicals:

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Agarose 50bp-50kbp
Universal Agarose from GeneON is ideal for use as standard agarose for analytical as well as preparative nucleic acid electrophoresis of fragments from 50 bp to 50 kbp. Even at low concentrations the gel produced is very firm
Proteinase K
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable
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