Proteinase K
Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable
| Cat.-no |
Description |
Amount |
Price € |
Shop |
| 405-002 |
Proteinase K |
200 mg |
79.00 |
add |
| 405-010 |
Proteinase K |
1000 mg |
175.00 |
add |
Proteinase K: Order/request by E-mail
Proteinase K: Datasheet
Proteinase K: Deutsche Beschreibung
Application:
- Digestion of proteins
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The Protein with a molecular weight 28.900 kD cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinase K is not inactivated by chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4-12.5).
Activity: > 30 units/mg protein (hemoglobin, pH 7.5, 37°C)
Unit definition:
Unit definition One unit is the amount of enzyme which releases at 37°C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
Features:
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is
strongly inhibited by PMSF.
Usage:
In presence of 0.5 – 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
Quality:
- purified by chromatography and lyophilised
- RNases: not detectable
- DNases: not detectable
- Exonucleases: not detectable
Storage:
4 °C or -20 °C for at least 24 months, shipment at ambient temperature
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