Cat.-no	Description	Amount	Price €	Shop
310005	Protein Marker PS11 (11 - 175 kDa)	1 x 500 µl	89.00	add
310006	Protein Marker PS11 (11 - 175 kDa)	5 x 500 µl	380.00	add

Protein Marker PS11: Order by E-mail
Protein Marker PS11: Datasheet
Protein Marker PS11: Deutsche Beschreibung

Description/Preparation:
Protein Marker PS11 contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The Protein Ladder allows to monitor molecular weight separation during electrophoresis, estimate molecular weights The marker is ready-to-use. There is no need to boil. Easy to identify: includes the ~10, ~40 and ~90 kDa reference bands coupled with an blue dye

Applications
• Monitoring of protein migration during SDS-
   polyacrylamide gel electrophoresis.
• Monitoring of protein transfer onto membranes
   during Western blotting.
• Sizing of proteins on SDS-polyacrylamide gels
   and Western blots.

Usage:  
Mini gel application: 5 μl/well; 2.5 µl per well blots
Standard gel application: 10 μl/well; 5 µl per well for blots

Number of bands: 11      10.5, 14, 22, 29, 42, 51, 62, 70, 95, 130, 175 kDa
 
Loading: 
Loading Denaturing Polyacrylamide gels (SDS-PAGE):
- Thaw marker at room temperature or heat at 37 –   40 °C for a few minutes. Do not boil!
- Vortex gently to ensure the solution is 
  homogeneous and load the ladder on SDS-
  polyacrylamide gel
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 10 μl per well for large gels, 5 μl per well for blots

1 ml marker is sufficient for 200 mini gels or 100 standard gels.
It is recommended to divide the marker into aliquots to avoid contamination of the stock solution.

Note: 
Protein Marker PS11 is optimized for runs on 15 % SDS polyacrylamide gels. 4 to 12 % gels may cause
proteins with low molecular weights to migrate with the dye front. On 12 to 15 % and gradient gels all bands are visible.

Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage: at -20°C

Shipment: on blue ice



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