SuperHot qPCR Master Mix RT

qPCR Master Mix RT: Datasheet
qPCR Master Mix: Deutsche Beschreibung

Features:
- The Master Mix (2X) offers both: high sensitivity and specificity
- Time saving ready-to-use qPCR Mastermix
- repeatable and reliable results
- efficient PCR for a wide range of template concentrations
- activation time < 3 min

Applications:
- Realtime PCR and quantitative PCR e.g. with Sybr green, Eva green or probes
- High-throughput PCR
- Multiplex PCR
- Low copy targets PCR

Description:
The Master Mix contains all reagents required for qPCR (except template and primer) in a premixed 2x concentrated ready-to-use solution. The high specificity and sensitivity of the mix is achieved by an optimized hot-start polymerase. Its activity is blocked at ambient temperature and switched on automatically at the onset of the initial denaturation. The thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during PCR setup.

Concentration: The Mastermix is 2x concentrated

List of components qPCR / RTD-PCR Master mix.
Hot-Start Polymerase (m-Superhot-Taq) for qPCR, dATP, dCTP, dGTP, dTTP, reaction buffer with  stabilizers and enhancers, 1 Tube PCR-grade water, 1 Tube MgCl₂

Quality control:
- Performance and purity tests
- Endodeoxyribonuclease Assay
- Real time PCR Test with SmartCycler II
- PCR Test with Lambda DNA (12 kb-fragment) and human placental DNA (3 kb-fragment)

Transportation: on blue ice

Storage: at 4°C for 3 months, at -20°C for more than 12 months

Note: 
-  an individual optimization of annealing temperature may be necessary for new combinations of primers
   and Template DNA