UDG Uracil DNA Glycosylase 200 units

Uracil-DNA Glycosylase (UDG): Datasheet

Applications:
- site-directed mutagenesis
- as a probe for protein-DNA interaction studies
- Glycosylase mediated single nucleotide polymorphism detection (GMPD) 
- SNP genotyping
- Rapid and efficient cloning of PCR products
- Elimination carry-over contamination in PCR

Description: 
The Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. Shows no activity on RNA. Molecular weight: 25.6 kDa monomer.

Concentration: 
high concentrated HC: 20 - 40 u/µl

Storage Buffer:
20 mM Tris-HCl (pH 8.0); 50 mM NaCl; 1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol

Reaction Buffer 10X
200 mM Tris-HCl (pH 8.0 at 25°C), 10 mM EDTA, 10 mM DTT. Incubation of 1X buffer at 37°C.

Unit definition:
One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [³H]-uracil in a 50 µl reaction containing 0,2 µg DNA (10⁴ – 10⁵ cpm/µg) in 30 minutes at 37°C.

Quality control:
Free of endodeoxyribonucleases, exodeoxyribonucleases, phosphatases and ribonucleases.

Usage:  
Treatment of 1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. 95 % of the enzyme can be inactivated by incubation at 95°C for 10 minutes.

Transportation: on blue ice

Storage: at -20°C for 24 months