Taq Polymerase Maximo 5x500 units

Maximo Taq DNA Polymerase: Datasheet

Features:
Maximo Taq DNA Polymerase provides robust PCR performance in a wide range of PCR applications and different templates. Best value in terms of cost per unit.

Applications:
- Standard / General PCR
- Multiplex PCR
- High-throughput PCR
- Primer extension
- Gene mutation
- T/A cloning

Description: 
Maximo Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.

Concentration:  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 50% Glycerol, 0.5% Nonident P40, 0.5% Tween 20

Reaction Buffers supplied with the enzyme:

10X Buffer I:  500mM KCl, 100mM Tris-HCl, pH 9.0, 1% Triton X-100, 15mM MgCl₂
10X Buffer II: 500mM KCl, 100mM Tris-HCl, p H 9.0. 1% Triton X-100
MgCl₂: 100 mM

Quality control: 
- PCR with various templates – genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation and level of bacterial DNA contamination
 
Transportation: on blue ice

Storage: at -20°C for 24 months

Usage: