Features - Yields up to 20µg of DNA - Multiple samples can be processed rapidly in less than 30 minutes - No organic-based extraction required - Highly pure plasmid DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, DNA sequencing, ligation, transformation, etc. Application For high quality plasmid DNA isolation. Message Plasmid DNA Extraction Kit is designed for rapid and efficient purification of high copy and low copy plasmid DNA without the need for precipitation or organic extractions. This it uses a specially-treated glass filter membrane fixed into a column to efficiently bind DNA in the presence of high salt. Combining alkaline lysis-SDS and minicolumn spin technology, up o 20μg of plasmid DNA from bacterial cultures can be isolated. Multiple samples can be processed rapidly and with practice, the purification takes less than 30 minutes. Optimized buffers ensure only highly pure plasmid DNA is extracted and is ready to use in many routine molecular biology applications such as restriction enzyme digestion, radioactive/fluorescence DNA sequencing, PCR, ligation, transformation and other manipulations.