m-Anti-Taq: Datasheet
Features:
- Anti-Taq can inhibit the reaction of Polymerases at room temperature
- Ideal as enhancer for PCR reactions
Description:
m-anti-Taq were derived from hybridoma. m-anti-Taq antibodies are mouse IgG type.
Application:
Polymerase detergent/enhancer for:
- High specific amplification
- Multiplex amplification
- High sensitivity applications
- Low-copy number PCR
Unit definition:
One unit is defined as the amount of antibodies required to blocks 50% activity of 1 µg of Polymerase
Note: The ratio units/mg of polymerase varies (up to factor of 10). The binding rate Anti-Taq depends strongly of the polymerization region (active epitopes) of the polymerase. The optimized mixture has to be found in empiric test and for every lot of polymerase, again.
Storage Buffer:
20mM Tris-HCl (pH 7.0, at 22oC);50 mM KCl;0.1mM EDTA; 50% glycerol
Concentration: 2-10 mg/ml
Units/mg-ratio:
2300 units of the specific polymerase activity are equal to 1 mg of antibodies
QC-Test:
> 95% of protein in SDS electrophoresis in 15% PAAG
Storage: at – 20°C for 24 months
Transportation: at room temperature or with blue ice
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