GeneOn :: DFS-Taq DNA Polymerase (DNA free) 20x500 units

DFS-Taq DNA Polymerase (DNA free) 20x500 units

DFS-Taq DNA Polymerase (DNA free) 20x500 units

 

Maximo Taq Polymerase DNA-free: Datasheet
Maximo Taq Polymerase DNA-free: Tests and Evaluation sheet


E coli free DFS Taq DNA Polymerase PCR3
Features:
Researchers are often encounting with contaminating DNA present in their polymerase preparations that often preclude or obscure accurate interpretation of PCR results, especially when targeting conserved sequences. Maximo DFS-Taq Polymerase is ideal in detecting and identifying bacterial DNA, looking for a more accurate method in mutation scanning techniques, or wanting to prevent the amplification of undesired DNA sequences.

Applications Taq Polymerase:
- Standard / General PCR
- PCR with bacterial DNA
- Quantitative PCR
- E. coli contamination studies
- Microbial (i.e., 16S/23S) contamination
  studies
- Forensic studies
- PCR cloning
- RT-PCR 

Description: 
Maximo Taq Polymerase is a recombinant thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94KD. The enzyme is free of DNA-contaminations.

Concentration:
  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.05 mM EDTA, 1 mM DTT, 50% glycerol.

Reaction Buffers supplied with Taq Polymerase:

10X Buffer I:
200mM Tris-HCl, 100 mM KCl, 100mM (NH4)2S04, 1% Triton X-100, at pH 8.8 (25°C)
10X Buffer II: 
200mM Tris-HCl, 100 mM KCl, 20mM MgSO4, 100mM (NH4)2S04, 1% Triton X-100, at pH 8.8 (25°C)
MgCl2: 25 mM
E coli free DFS Taq DNA Polymerase PCR1



Quality control: 
- E. coli genomic DNA testing after
  treatment  with T5 DNAse
- PCR with various templates – human and 
  bovine
  genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation 
 
Transportation: 
on blue ice


Storage:
at -20°C for 12 months




Usage:  

 

Components Volume per reaction
 10X reaction buffer I  5 µl
 25 mM MgCl2  1.5 µl (if necessary)
 dNTP-Mix (40mM)  1.0 µl
 Up-stream primer (10 µM stock)  0,5-2.5 µl
 Down-stream primer (10µM stock)   0.5-2,5 µl
 Template DNA  0.1-15 ng/ml plasmid DNA
 1-10 µg/ml genomic DNA
 Maximo Taq DNA Polymerase (5 u/µl)   0.2 - 1.0 µl  
 Sterile dest. Water (molecular grade)  up to 50 µl total reaction volume


Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl2 concentration for best result

General Thermo-Cycler protocol:

 Step  Time  Temperature
 Initial denaturation  2-5 min  94-95°C
 
25-30 Cycles:
 Denaturation
 Annealing
 Extension
 
 10-25 sec
 10-25 sec
 60 sec

 94-95°C
 55-65°C
 72°C per 1kb
 
 Final extension    5 min  72°C
Product code/no: N107  
 
Your Shop-price: 899,00
31%
Market price: € 1.299,00
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