GeneOn :: DFS-Taq DNA Polymerase (DNA free) 20x500 units

DFS-Taq DNA Polymerase (DNA free) 20x500 units

DFS-Taq DNA Polymerase (DNA free) 20x500 units


Maximo Taq Polymerase DNA-free: Datasheet
Maximo Taq Polymerase DNA-free: Tests and Evaluation sheet

E coli free DFS Taq DNA Polymerase PCR3
Researchers are often encounting with contaminating DNA present in their polymerase preparations that often preclude or obscure accurate interpretation of PCR results, especially when targeting conserved sequences. Maximo DFS-Taq Polymerase is ideal in detecting and identifying bacterial DNA, looking for a more accurate method in mutation scanning techniques, or wanting to prevent the amplification of undesired DNA sequences.

Applications Taq Polymerase:
- Standard / General PCR
- PCR with bacterial DNA
- Quantitative PCR
- E. coli contamination studies
- Microbial (i.e., 16S/23S) contamination
- Forensic studies
- PCR cloning

Maximo Taq Polymerase is a recombinant thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94KD. The enzyme is free of DNA-contaminations.

  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.05 mM EDTA, 1 mM DTT, 50% glycerol.

Reaction Buffers supplied with Taq Polymerase:

10X Buffer I:
200mM Tris-HCl, 100 mM KCl, 100mM (NH4)2S04, 1% Triton X-100, at pH 8.8 (25°C)
10X Buffer II: 
200mM Tris-HCl, 100 mM KCl, 20mM MgSO4, 100mM (NH4)2S04, 1% Triton X-100, at pH 8.8 (25°C)
MgCl2: 25 mM
E coli free DFS Taq DNA Polymerase PCR1

Quality control: 
- E. coli genomic DNA testing after
  treatment  with T5 DNAse
- PCR with various templates – human and 
  genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation 
on blue ice

at -20°C for 12 months



Components Volume per reaction
 10X reaction buffer I  5 µl
 25 mM MgCl2  1.5 µl (if necessary)
 dNTP-Mix (40mM)  1.0 µl
 Up-stream primer (10 µM stock)  0,5-2.5 µl
 Down-stream primer (10µM stock)   0.5-2,5 µl
 Template DNA  0.1-15 ng/ml plasmid DNA
 1-10 µg/ml genomic DNA
 Maximo Taq DNA Polymerase (5 u/µl)   0.2 - 1.0 µl  
 Sterile dest. Water (molecular grade)  up to 50 µl total reaction volume

- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl2 concentration for best result

General Thermo-Cycler protocol:

 Step  Time  Temperature
 Initial denaturation  2-5 min  94-95°C
25-30 Cycles:
 10-25 sec
 10-25 sec
 60 sec

 72°C per 1kb
 Final extension    5 min  72°C
Product code/no: N107  
Your Shop-price: 899,00
Market price: € 1.299,00

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