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PCR-Diagnostik-Mastermixe » PCR-Nachweistest für DNA
PCR-Nachweistest für DNA
Detektion von Infektionskrankheiten mit gefriergetrockneten PCR Mastermixen
Beschreibung:
Vor der Analyse wird die DNA aus dem biologischen Material mit einem geeigneten Aufreinigungssystem isoliert. Es gibt keine Einschränkungen bei des Wahl des Aufreinigungssystems.
Geeignetes Probenmaterial:
-Speichel, Urin, Tränenflüssigkeit, Schleim und andere Bioproben mit geringen Konzentrationen an Proteinen, Polysacchariden und Fette.
- Blut, Gewebe, Zellsuspensionen und andere Bioproben mit hohen Konzentrationen an Proteinen Polysacchariden und Fette.
PCR-Detektions Testsystem: Datenblatt
PCR-detection test system: English description
PCR-Ansatz (total 20 µl):
- max. 10 µl aufgereinigte DNA
- max. 10 µl des mitgelieferten Dilutions-Puffer
- optional mit PCR-Grade dest. Wasser auf 20 µl Endvolumen auffüllen
- gut mischen
Standard-Protokoll:
| Schritt |
Zeit |
Temperatur |
| Erste Denaturierung |
2-3 Min |
95°C |
45 Zyklen:
Denaturierung
Annealing
Extension
|
20-25 Sec
20-40 Sec
60 Sec |
95°C
58-62°C *1)
74°C
|
| Schluss-Extension |
2-3 Min |
74°C |
*1) Genaue Temperatur im Manual
Auswertung: im Agarose Gel
Hinweis: Die gefriergetrockneten Mastermixe sind ausschließlich für Forschungszwecke bestimmt und sind nicht CE-zertifizert!
Transport: bei Raumtemperatur
Lagerung: bei Raumtemperatur oder für mehr als 12 Monate bei 2-15°C
| Product code |
Description |
Short-cut |
Temp. |
Ampl.size
(bp)
|
Price € |
shop |
| G10 |
Adeno-associated virus, type 2 |
AAV-2 |
58°C |
450 |
399.00 |
add |
| G20 |
Adenovirus, general |
AdV gen |
56°C |
272 |
399.00 |
add |
| G30 |
Adenovirus, genotypes |
AdV types |
60°C |
272 |
399.00 |
add |
| G40 |
Aleutian mink disease parvovirus |
AMDV |
58°C |
298 |
399.00 |
add |
| G50 |
Aspergilus fumigatus |
Afu |
58°C |
456 |
399.00 |
add |
| G60 |
Babesia canis |
Bca |
58°C |
359 |
399.00 |
add |
| G70 |
Babesia divergens |
Bdi |
58°C |
359 |
399.00 |
add |
| G80 |
Babesia equi |
Beq |
58°C |
362 |
399.00 |
add |
| G90 |
Babesia microti |
Bmi |
58°C |
362 |
399.00 |
add |
| G100 |
Babesia spp. |
Bab |
58°C |
359 |
399.00 |
add |
| G110 |
Bacillus anthracis |
Ban |
58°C |
320 |
399.00 |
add |
| G120 |
Bacteroides fragilis |
Bfr |
58°C |
535 |
399.00 |
add |
| G130 |
Bordetella pertussis |
Bpe |
58°C |
279 |
399.00 |
add |
| G140 |
Borrelia afzelii |
Baf |
58°C |
445 |
399.00 |
add |
| G150 |
Borrelia burgdorferi |
Bbu |
58°C |
445 |
399.00 |
add |
| G160 |
Borrelia garinii |
Bga |
58°C |
445 |
399.00 |
add |
| G170 |
Borrelia spp.(burgdof.+garinii+afzelii) |
Bor |
58°C |
445 |
399.00 |
add |
| G180 |
Bovine herpes virus,I type |
BHV I |
58°C |
355 |
399.00 |
add |
| G190 |
Bovine immunodeficiency virus |
proBIV |
58°C |
382 |
399.00 |
add |
| G200 |
Bovine leukemia virus (gag) |
proBLV |
58°C |
347 |
399.00 |
add |
| G210 |
Brucella melitensis. |
Bme. |
62°C |
442 |
399.00 |
add |
| G220 |
Campylobacter jejuni |
Cje |
58°C |
364 |
399.00 |
add |
| G230 |
Candida albicans |
Cal |
58°C |
490 |
399.00 |
add |
| G240 |
Candida glabrata |
Cgl |
58°C |
295 |
399.00 |
add |
| G250 |
Chlamydia pneumoniae |
Cpn. |
58°C |
448 |
399.00 |
add |
| G260 |
Chlamydia psittaci |
Cps. |
58°C |
356 |
399.00 |
add |
| G270 |
Chlamydia spp. |
Chl. |
58°C |
560 |
399.00 |
add |
| G280 |
Chlamydia trachomatis |
Ctr |
58°C |
410 |
399.00 |
add |
| G290 |
Clostridium difficile |
Cdi |
58°C |
517 |
399.00 |
add |
| G300 |
Cryptococcus neoformans |
Cne |
58°C |
307 |
399.00 |
add |
| G310 |
Cryptosporidium parvum |
Cpa |
58°C |
475 |
399.00 |
add |
| G320 |
Ehrlichia murris/Yamaguchi |
Emu |
58°C |
223 |
399.00 |
add |
| G330 |
Ehrlichia spp. |
Ehr |
58°C |
337 |
399.00 |
add |
| G340 |
Ehrlichia(Anaplasma) phagocytophilum |
Epf |
58°C |
227 |
399.00 |
add |
| G350 |
Entamoeba histolitica |
Ehi |
58°C |
887 |
399.00 |
add |
| G360 |
Enterococcus faecalis |
Efal |
58°C |
499 |
399.00 |
add |
| G370 |
Enterococcus faecium |
Efam |
58°C |
541 |
399.00 |
add |
| G380 |
Epstein-Barr virus |
EBV |
58°C |
558 |
399.00 |
add |
| G390 |
Erythromycin resistance gene, ermF |
ermF |
58°C |
492 |
399.00 |
add |
| G400 |
Escherichia coli,CFT07, uropathogenic |
Eco |
58°C |
606 |
399.00 |
add |
| G410 |
Francisella tularensis |
Ftu |
58°C |
333 |
399.00 |
add |
| G420 |
Gardnerella vaginalis |
Gva. |
58°C |
424 |
399.00 |
add |
| G430 |
Giardia lamblia |
Gla |
58°C |
318 |
399.00 |
add |
| G440 |
Haemophilus influenzae |
Hin |
58°C |
381 |
399.00 |
add |
| G450 |
Helicobacter pylori (cagA) |
Hpy (cagA) |
58°C |
682 |
399.00 |
add |
| G460 |
Helicobacter pylori (ure) |
Hpy (ure) |
58°C |
318 |
399.00 |
add |
| G470 |
Helicobacter pylori (vacA) |
Hpy (vacA) |
58°C |
565 |
399.00 |
add |
| G480 |
Hepatitis B virus |
HBV |
58°C |
473 |
399.00 |
add |
| G490 |
Herpes simplex virus 1 type |
HSV 1 |
58°C |
331 |
399.00 |
add |
| G500 |
Herpes simplex virus 1/2 types |
HSV 1/2 |
58°C |
360 |
399.00 |
add |
| G510 |
Herpes simplex virus 2 type |
HSV 2 |
58°C |
432 |
399.00 |
add |
| G520 |
Human papillomavirus, low risk |
HPV l.r. |
58°C |
450 |
399.00 |
add |
| G530 |
Human cytomegalovirus |
HCMV |
58°C |
451 |
399.00 |
add |
| G540 |
Human herpes virus 6 type |
HHV 6 |
58°C |
371 |
399.00 |
add |
| G550 |
Human herpes virus 7 type |
HHV 7 |
58°C |
437 |
399.00 |
add |
| G560 |
Human herpes virus 8 type |
HHV 8 |
58°C |
272 |
399.00 |
add |
| G570 |
Human immunodeficiency virus 1 |
proHIV I |
58°C |
376 |
399.00 |
add |
| G580 |
Human papillomavirus, 16 type |
HPV 16 |
60°C |
692 |
399.00 |
add |
| G590 |
Human papillomavirus, 16/18 types |
HPV16/18 |
60°C |
692 |
399.00 |
add |
| G600 |
Human papillomavirus, 18 type |
HPV 18 |
60°C |
692 |
399.00 |
add |
| G610 |
Human papillomavirus, general |
HPVgen. |
56°C |
450 |
399.00 |
add |
| G620 |
Human papillomavirus, high risk |
HPV h.r. |
58°C |
450 |
399.00 |
add |
| G630 |
Human papillomavirus, high risk-plus |
HPV h.r.+ |
58°C |
692 |
399.00 |
add |
| G640 |
Human papillomavirus,genotyping |
HPV typing |
60°C |
450 |
399.00 |
add |
| G650 |
HumanT-lymphocytotropic virus 1/II |
proHTLV I/II |
60°C |
598 |
399.00 |
add |
| G660 |
Klebsiella pneumoniae |
Kpn |
58°C |
541 |
399.00 |
add |
| G670 |
Lactobacillus spp. |
Lac |
58°C |
239 |
399.00 |
add |
| G680 |
Legionella pneumophila |
Lpn |
58°C |
511 |
399.00 |
add |
| G690 |
Leptospira interrogans |
Lin. |
58°C |
668 |
399.00 |
add |
| G700 |
Leptospira spp. (pathogenic serovars) |
Lep. |
58°C |
423 |
399.00 |
add |
| G710 |
Listeria monocytogenes |
Lmo |
58°C |
226 |
399.00 |
add |
| G720 |
Listeria spp. |
Lis |
58°C |
429 |
399.00 |
add |
| G730 |
Mobiluncus curtisii |
Mcu |
58°C |
224 |
399.00 |
add |
| G740 |
Moraxella catarrhalis |
Mca |
58°C |
550 |
399.00 |
add |
| G750 |
Morganella morganii |
Mmo |
58°C |
356 |
399.00 |
add |
| G760 |
Mycobacterium avium,subspp paratubercul. |
Mav |
62°C |
268 |
399.00 |
add |
| G770 |
Mycobacterium intracellulare |
Min |
62°C |
234 |
399.00 |
add |
| G780 |
Mycobacterium tuberculosis |
Mtu. |
62°C |
222 |
399.00 |
add |
| G790 |
Mycobacterium tuberculosis+bovis |
M(tu+bo) |
62°C |
211 |
399.00 |
add |
| G800 |
Mycoplasma hominis |
Mho. |
58°C |
282 |
399.00 |
add |
| G810 |
Mycoplasma pneumoniae |
Mpn. |
58°C |
316 |
399.00 |
add |
| G820 |
Mycoplasma fermentans |
Mfe |
58°C |
700 |
399.00 |
add |
| G830 |
Mycoplasma genitalium |
Mge |
58°C |
538 |
399.00 |
add |
| G840 |
Mycoplasma penetrans |
Mpe |
58°C |
318 |
399.00 |
add |
| G850 |
Mycoplasma spp.(Mge+Mho+Mpn+Mfe+Mpe) |
Myc |
58°C |
316 |
399.00 |
add |
| G860 |
Neisseria gonorrhoea |
Ngo |
58°C |
591 |
399.00 |
add |
| G870 |
Parvovirus B19 |
PV B19 |
58°C |
743 |
399.00 |
add |
| G880 |
Peotostreptococcus anaerobius |
Pan |
58°C |
396 |
399.00 |
add |
| G890 |
Plasmodium falciparum |
Pfa |
58°C |
319 |
399.00 |
add |
| G900 |
Plasmodium malariae |
Pma |
58°C |
307 |
399.00 |
add |
| G910 |
Plasmodium ovale |
Pov |
58°C |
312 |
399.00 |
add |
| G920 |
Plasmodium spp.(Pfa+Pvi+Pov+Pma) |
Pla |
58°C |
319 |
399.00 |
add |
| G930 |
Plasmodium vivax |
Pvi |
58°C |
310 |
399.00 |
add |
| G940 |
Pneumocystis carinii |
Pca. |
58°C |
396 |
399.00 |
add |
| G950 |
Poliomavirusis (JCV, BKV, SV40) |
JCV,BKV,SV |
58°C |
232 |
399.00 |
add |
| G960 |
Prevotella bivia |
Pbi |
58°C |
279 |
399.00 |
add |
| G970 |
Proteus mirabilis |
Pmi |
58°C |
448 |
399.00 |
add |
| G980 |
Pseudomonas aeruginosa |
Pae |
58°C |
254 |
399.00 |
add |
| G990 |
Rickettsia spp. |
Ric |
58°C |
259 |
399.00 |
add |
| G1000 |
SEN virus D |
SENV-D |
58°C |
346 |
399.00 |
add |
| G1010 |
SEN virus D/H |
SENV-D/H |
58°C |
346 |
399.00 |
add |
| G1020 |
SEN virus H |
SENV-H |
58°C |
346 |
399.00 |
add |
| G1030 |
Streptococcus agalactiae |
Sag. |
58°C |
583 |
399.00 |
add |
| G1040 |
Streptococcus pneumoniae |
Spn |
58°C |
567 |
399.00 |
add |
| G1050 |
Streptococcus pyogenes |
Spy |
58°C |
609 |
399.00 |
add |
| G1060 |
Tetracycline resistance gene, tetM |
tetM |
58°C |
430 |
399.00 |
add |
| G1070 |
Tetracycline resistance gene, tetQ |
tetQ |
58°C |
392 |
399.00 |
add |
| G1080 |
Toxoplasma gondii |
Tgo |
58°C |
523 |
399.00 |
add |
| G1090 |
Transfusion transmit.virus,general |
TTVgen. |
58°C |
276 |
399.00 |
add |
| G1100 |
Transfusion transmitted.virus, types |
TTV 1a,1b,2,3 |
60°C |
415, … |
399.00 |
add |
| G1110 |
Treponema pallidum |
Tpa. |
58°C |
325 |
399.00 |
add |
| G1120 |
Trichomonas vaginalis |
Tva. |
58°C |
771 |
399.00 |
add |
| G1130 |
Ureaplasma parvum, biovar 1 |
Upa. |
58°C |
540 |
399.00 |
add |
| G1140 |
Ureaplasma (parvum+ urealytic.) |
Ure. |
58°C |
554 |
399.00 |
add |
| G1150 |
Ureaplasma urealyticum, biovar2 |
Uur. |
58°C |
435 |
399.00 |
add |
| G1160 |
Varicella-zoster virus |
VZV |
58°C |
381 |
399.00 |
add |
| G1170 |
Vibrio cholerae (tox R) |
Vcho (toxR) |
58°C |
624 |
399.00 |
add |
| G1180 |
Vibrio cholerae (îmð W) |
Vcho (împW) |
58°C |
441 |
399.00 |
add |
| G1190 |
Yersinia enterocolitica |
Yen |
58°C |
373 |
399.00 |
add |
| G1200 |
Yersinia pestis |
Ype |
58°C |
351 |
399.00 |
add |
| G1210 |
Yersinia pseudotuberculesis |
Yps |
58°C |
272 |
399.00 |
add |
Verwandte Produkte:
Agarose für die Gelelektrophorese
DNA Marker und Standards
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