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DNA Sizer/Marker Classic » pBR322 DNA/Hinf I digest ready-to-use
pBR322 DNA/Hinf I digest ready-to-use
DNA Sizer: pBR322 DNA/Hinf I digest is ideal for qualitative analysis of dsDNA
| Cat.-no |
Description |
Amount |
Price € |
Shop |
| 300040 |
pBR322 DNA/Hinf I |
2 x 100 µg |
110.00 |
add |
| 300041 |
BR322 DNA/Hinf I |
5 x 100 µg |
490.00 |
add |
pBR322 DNA/Hinf I: Order E-mail:
pBR322 DNA/Hinf I: Datasheet
pBR322 DNA/Hinf I: Deutsche Beschreibung
Features: Supplied in 6X Loading buffer
Description/Preparation:
The pBR322 DNA/Hinf I digest yields the 10 fragments. pBR322 DNA was completely digested by Hinf I,
phenol/chloroform extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Usage: 0,5 µg/lane
Concentration: 0,4 µg/µl
Number of bands: 10 1632, 517, 504, 396, 344, 298, 221, 220, 154, 75
Loading:
Agarose Gel / Polyacrylamide Gel
- Vortex gently before using
- apply 0,5 µg (agarose) or 0.5-0.9 µg (polyacrylamide gel) per 1 mm lane
Quantification:
See the graph for the percentage of the bands per band in ng, relating to 0.5 μg loaded marker. Use the same volume of DNA and marker. Additionally the concentration of loading buffer in samples and marker should be equal.
Note:
Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
Transportation: Shipped on blue ice or room temperature
Storage: at -20°C for 24 months
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