Cat.-no	Description	Amount	Price €	Shop
S108	p- Taq Polymerase (qPCR)	200 units	65.00	add
S109	p- Taq Polymerase (qPCR)	5x200 units	259.00	add

p- Taq Polymerase: Order/request by E-mail
p- Taq Polymerase: Datasheet
p- Taq Polymerase: Deutsche Beschreibung

Features: p- Taq Polymerase for qPCR is designed for Real Time PCR. Polyclonal antibodies inhibit the reaction at room temperature until after the first denaturation step. This prevents primer-dimers and other artefacts. Using the enzyme there is no need to adjust the existing standard PCR protocol in your lab.

Applications for Taq Polymerase:
- Hot Start and real time PCR
- Multiplex PCR
- Amplification of complex genomic and cDNA templates
- no primer-dimers and other artefacts; inactive at room temperature
- short activation time
- enhanced PCR sensitivity

Description:
Maximo p- Taq Polymerase is an optimized mixture of a high processive Taq Polymerase and polyclonal antibodies to Taq Polymerase. The enzyme is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa.
 
Concentration: 5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30 min at 72°C degree

Storage Buffer: 
50 mM Tris-HCl, pH 8.2, 0.1 mM EDTA, 0.1% Tween 20, 0.1% NP40, 1 mM DTT, 50% Glycerol

Reaction Buffer:
10X Buffer: 100mM KCl, 80mM (NH₄)₂SO₄, 100mM Tris-HCl, pH9.0, 0.5% Nonident P40, 15 mM MgSO₄

Quality control for Taq Poymerase:
Activity and performance test, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test
 
Usage:  
Use your existing and optimized protocol. In contrast to chemically modified Taq DNA pol. where the first denaturation step needs up to 15 min, Maximo p-Superhot Taq does not need a prolonged heating or denaturation time.

Components	Volume per reaction
10X reaction buffer	5 µl
Mg+	optional
dNTP-Mix (40mM)	1.0 µl
Up-stream primer (10 µM stock)	0,5-2.5 µl
Down-stream primer (10µM stock)	0.5-2,5 µl
Template DNA	0.1-15 ng/ml plasmid DNA  1-10 µg/ml genomic DNA
Maximo p Taq Polymerase (5 u/µl)	0.2 - 1.0 µl
Sterile dest. Water (molecular bio. grade)	up to 50 µl total reaction volume

Note: 
- vortex all solutions carefully before using
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	4-5 min	94-95°C
25-30 Cycles:  Denaturation  Annealing  Extension	10-25 sec  10-25 sec  60 sec	94-95°C  55-65°C  72°C per 1kb
Final extension	5 min	72°C

Note: 
- In case of low amount of DNA template, additionally cycles may be used 

Transportation: on blue ice

Storage: Taq Polymerase can be stored at -20°C for 24 months

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