Catalog-no	Description	units	Euro	Order by E-mail	Shop
250-124S	NotI	300 u	18.00	NotI 300 u	Add
250-124L	NotI	5x300 u	90.00	NotI 1500 u	Add

Overhang: 5´ - GGCC


Cut Site:

G C / G G C C G C
C G C C G G / C G

---

Source: Nocardia otitidis-caviarum.

Buffer supplied: 10x Not I.
 
Substrate for unit definition: Adenovirus-2 DNA.
 
Reaction conditions: 150 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl₂, 0.1% Triton X-100, 100 μg/ml BSA. Incubate at 37°C.
 
Storage buffer:  200 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA and 50% glycerol.

Store: at -20°C.
 
Absence of contaminants: Fifty units of NotI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Adeno-2 DNA at 37°C. After 25-fold overdigestion with NotI, more than 98% of the DNA fragments can be ligated and recut with this enzyme.
 
Heat inactivation: 65°C for 20 minutes.

Note: Activity may be blocked by overlapping CpG methylation super coiled plasmids may require up to
5 –fold more NotI for complete digestion than linear DNAs.

Table of Restriction Endonucleases

Alu I - BseC I	BshF I - Hpa I	Kpn I - Pvu II	Rsa I - SnaB I	SseB I - Xba I
Alu I	BshF I	Kpn I	Rsa I	SseB I
ApaL I	BsiS I (N/A)	Mbo I	Sal I	Sst I
Asu II	BssA I	MspC I	Sau3A I	Sty I
BamH I	BstE II	Nae I	Sca I	Taq I
Bcl I	CspA I	Nco I	Sfi I	Xba I
Bgl I	EcoR I	Nhe I	SgrB I (Sac II)
Bgl II	EcoR V	Not I	Sla I
BseA I	Hind III	Nru I	Sma I
BseB I	Hinf I	Pst I	SnaB I
BseC I	Hpa I	Pvu II	Sph I

Table of Isoschizomeres

Isosch.	Link to Enzmye	Isosch.	Link to Enzmye	Isosch.	Link to Enzmye	Isosch.	Link to Enzmye
Aat I	SseB I	BsiX	BseC I	Cbi I	Asu II	NgoM IV	Nae I
Acc III	BseA I	BIsp106 I	BseC I	Cfr42 I	SgrB I	NspV	Asu II
Afi II	MspC I	Bsp119 I	Asu II	Cla I	BseC I	PaeR7 I	Sla I
Age I	CspA I	Bsp13 I	BseA I	Csp45 I	Asu II	Pal I	BshF I
AsiA II	CspA I	BspD I	BseC I	Eco147 I	SseB I	PinA I	CspA I
Ban III	BseC I	BspE I	BseA I	EcoR II	BseB I	Pm55 II	SseB I
Bfr I	MspCI	BspT 1	MspC I	Hae III	BshF I	PspG I	BseB I
Bpu14 I	Asu II	BspX I	BseC I	Kpn I	BseA I	Sac II	SgrB I
Bsa29 I	BseC I	Bst2U I	BseB I	Ksp I	SgrB I	Sfr274 I	Sla I
Bsc I	BseC I	Bst98 I	MspC I	Lsp I	Asu II	Sfr303 I	SgrB I
Bsh I	BshF I	BstB I	Asu II	Mro I	BseA I	Sfu I	Asu II
Bsh T I	CspA I	BstN I	BseB I	MroN I	Nae I	Sst II	SgrB I
BsiC I	Asu II	BstO I	BseB I	Msp I	BsiS I	Stu I	SseB I
BsiL I	BseB I	Bsu15 I	BseC I	Mva I	BseB I	Tlil	Sla I
BsiM I	BseA I	BsuR I	BshF I	NgoA IV	Nae I	Xho I	SseB I

 

 

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