Catalog-no	Description	units	Euro	Order by E-mail	Shop
250-146S	NheI	550 u	18.00	NheI 550 u	Add
250-146L	NheI	5x550 u	90.00	NheI 2750 u	Add

Overhang: 5´ - CTAG


Cut Site:

G / C T A G C
C G A T C / G
 

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Buffer supplied: 10x A

Substrate for unit definition:  λ DNA (Hind III digest).

Reaction conditions: 50 mM potassium acetate, 20 mM Tris-acetate (pH 7.9 @ 25°C),  10 mM magnesium acetate, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37^oC.

Storage buffer: 50 mM KCl, 10 mM  Tris-HCl (pH 7.5),  0.1 mM EDTA, 1 mM dithiothreitol,  200 μg/ml BSA and 50% glycerol.

Store: at -20^oC.

Absence of contaminants:  80 units of NheI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA/HindIII digest at 37^oC.  After 100-fold overdigestion with NheI, greater than 98% of the DNA fragments can be ligated and recut.

Heat inactivation: 65°C for 20 minutes.

Note: Activity inhibited by salt concentrations >100mM. Cleaves to leave a 5’ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI, or XbaI.

Star activity:  Low salt, high glycerol (>5%) concentrations, pH >8.0 or large excess of the enzyme may result in star activity.   

Note: Activity may be blocked by some combinations overlapping CpG methylation.

Table of Restriction Endonucleases

Alu I - BseC I	BshF I - Hpa I	Kpn I - Pvu II	Rsa I - SnaB I	SseB I - Xba I
Alu I	BshF I	Kpn I	Rsa I	SseB I
ApaL I	BsiS I (N/A)	Mbo I	Sal I	Sst I
Asu II	BssA I	MspC I	Sau3A I	Sty I
BamH I	BstE II	Nae I	Sca I	Taq I
Bcl I	CspA I	Nco I	Sfi I	Xba I
Bgl I	EcoR I	Nhe I	SgrB I (Sac II)
Bgl II	EcoR V	Not I	Sla I
BseA I	Hind III	Nru I	Sma I
BseB I	Hinf I	Pst I	SnaB I
BseC I	Hpa I	Pvu II	Sph I

Table of Isoschizomeres

Isosch.	Link to Enzmye	Isosch.	Link to Enzmye	Isosch.	Link to Enzmye	Isosch.	Link to Enzmye
Aat I	SseB I	BsiX	BseC I	Cbi I	Asu II	NgoM IV	Nae I
Acc III	BseA I	BIsp106 I	BseC I	Cfr42 I	SgrB I	NspV	Asu II
Afi II	MspC I	Bsp119 I	Asu II	Cla I	BseC I	PaeR7 I	Sla I
Age I	CspA I	Bsp13 I	BseA I	Csp45 I	Asu II	Pal I	BshF I
AsiA II	CspA I	BspD I	BseC I	Eco147 I	SseB I	PinA I	CspA I
Ban III	BseC I	BspE I	BseA I	EcoR II	BseB I	Pm55 II	SseB I
Bfr I	MspCI	BspT 1	MspC I	Hae III	BshF I	PspG I	BseB I
Bpu14 I	Asu II	BspX I	BseC I	Kpn I	BseA I	Sac II	SgrB I
Bsa29 I	BseC I	Bst2U I	BseB I	Ksp I	SgrB I	Sfr274 I	Sla I
Bsc I	BseC I	Bst98 I	MspC I	Lsp I	Asu II	Sfr303 I	SgrB I
Bsh I	BshF I	BstB I	Asu II	Mro I	BseA I	Sfu I	Asu II
Bsh T I	CspA I	BstN I	BseB I	MroN I	Nae I	Sst II	SgrB I
BsiC I	Asu II	BstO I	BseB I	Msp I	BsiS I	Stu I	SseB I
BsiL I	BseB I	Bsu15 I	BseC I	Mva I	BseB I	Tlil	Sla I
BsiM I	BseA I	BsuR I	BshF I	NgoA IV	Nae I	Xho I	SseB I

 

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