NaeI

Sequence of Nae I: 5´...GCCGGC...3´ 3´...CGGCCG...5´

Catalog-no	Description	units	Euro	Order by E-mail	Shop
250-122S	Nae I	300 u	18.00	Nae I 300 u	Add
250-122L	Nae I	5x300 u	90.00	Nae I 1500 u	Add

NaeI: Datasheet
NaeI: Order by E-Mail

Overhang: 5´ - blunt

Cut Site:

G C C / G G C
C G G / C C G

Isoschizomers: Pdi I

Neoschizomers: MroN I, NgoM IV

Source: Nocardia aerocolomigensis

Substrate for unit definition: pBR322.

Reaction conditions: 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C.

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol.

Store: at -20°C.

Absence of contaminants: Fifty units of NaeI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After ten-fold overdigestion with NaeI, greater than 80% of the DNA fragments can be ligated and recut with this enzyme.

Heat inactivation: 65°C for 20 minutes.

Note: Activity may be blocked by CpG methylation NaeI exhibits site preferences. pBR322 contains four NaeI recognition sequences. Two of these sites are easily cleaved, one is cleaved moderately slowly, the forth is cleaved 50-fold more slowly.

Table of Restriction Endonucleases

Alu I - BseC I BshF I - Hpa I Kpn I - Pvu II Rsa I - SnaB I SseB I - Xba I

Alu I BshF I Kpn I Rsa I SseB I

ApaL I BsiS I (N/A) Mbo I Sal I Sst I

Asu II BssA I MspC I Sau3A I Sty I

BamH I BstE II Nae I Sca I Taq I

Bcl I CspA I Nco I Sfi I Xba I

Bgl I EcoR I Nhe I SgrB I (Sac II)

Bgl II EcoR V Not I Sla I

BseA I Hind III Nru I Sma I

BseB I Hinf I Pst I SnaB I

BseC I Hpa I Pvu II Sph I

Table of Isoschizomeres

Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye

Aat I SseB I BsiX BseC I Cbi I Asu II NgoM IV Nae I

Acc III BseA I BIsp106 I BseC I Cfr42 I SgrB I NspV Asu II

Afi II MspC I Bsp119 I Asu II Cla I BseC I PaeR7 I Sla I

Age I CspA I Bsp13 I BseA I Csp45 I Asu II Pal I BshF I

AsiA II CspA I BspD I BseC I Eco147 I SseB I PinA I CspA I

Ban III BseC I BspE I BseA I EcoR II BseB I Pm55 II SseB I

Bfr I MspCI BspT 1 MspC I Hae III BshF I PspG I BseB I

Bpu14 I Asu II BspX I BseC I Kpn I BseA I Sac II SgrB I

Bsa29 I BseC I Bst2U I BseB I Ksp I SgrB I Sfr274 I Sla I

Bsc I BseC I Bst98 I MspC I Lsp I Asu II Sfr303 I SgrB I

Bsh I BshF I BstB I Asu II Mro I BseA I Sfu I Asu II

Bsh T I CspA I BstN I BseB I MroN I Nae I Sst II SgrB I

BsiC I Asu II BstO I BseB I Msp I BsiS I Stu I SseB I

BsiL I BseB I Bsu15 I BseC I Mva I BseB I Tlil Sla I

BsiM I BseA I BsuR I BshF I NgoA IV Nae I Xho I SseB I

>>> Service pages in English <<< >>> Serviceseiten auf Deutsch <<<