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You are here : PCR / DNA AmplificationPolymerases ╗ Hot-Start PCR: M-Superhot Taq Polymerase

Hot-Start PCR: M-Superhot Taq Polymerase

Superior Taq Polymerase for Real Time PCR and Hot-Start PCR, low-copy number PCR, PCR of difficult templates, Hot-Start activity (only 5 min initial denaturation). The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. The enzyme provides a convenient setting up at room temperature

 
 
Cat.-no Description Amount Price € Shop
 S105  Maximo M-Superhot Taq DNA Pol. (qPCR)     200 units     69.00
   55.00
 add 
 S106  Maximo M-Superhot Taq DNA Pol. (qPCR)  5x200 units  279.00
  price drop199.00
 add 
 S107  Maximo M-Superhot Taq DNA Pol. (qPCR)    5000 units  995.00
 895.00
 add

m-Superhot Taq DNA Polymerase for real-time PCR and Hot-Start PCR: Order/request by E-mail

m-Superhot Taq DNA Polymerase for real time PCR and Hot-start PCR: Datasheet

m-Superhot Taq DNA Polymerase for real-time PCR and Hot start PCR: Deutsche Beschreibung


Features: Maximo M-Superhot Taq DNA Polymerase for qPCR is designed for Real-Time PCR and Hot-start PCR. A special inhibitor suppresses the reaction at room temperature until after the first denaturation step. This prevents primer-dimers and other artefacts. Using the enzyme there is no need to adjust the existing standard PCR protocol.

Applications: 
- Hot Start and real time PCR
- Multiplex PCR
- Amplification of complex genomic and cDNA templates
- no primer-dimers and other artêfacts; inactive at room temperature
- short activation time for real time PCR
- enhanced PCR sensitivity

Description:
Maximo M-Superhot Taq DNA for qPCR and Hot-Start-PCR is an optimized mixture of a high processive Taq DNA Polymerase and special inhibitors to Taq DNA for real time PCR. The enzyme is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-stranded specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94kDa. It is developed for real time PCR or as basis enzyme for  real time PCR diagnostics systems.
 
Concentration: 5 u/µl

Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP's into acid-insoluble form in 30 minutes at 74oC under assay conditions:
25mM TAPS pH 9.3 at 25oC, 50mM KCl, 2mM MgCl2; 1mM beta-mercaptoethanol; 200µM each dATP, dGTP, dTTP and 100 µM dCTP (a mix of unlabled and µ-[32P]-labled); 12.5 µg activated salmon sperm DNA in the final volume of 50 µl

Storage Buffer:
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA; 1 mM DTT, 50 % Glycerol, 0.5 % Nonident P-40, 0.5 % Tween-20

Reaction Buffer:
Reaction buffer (10X)” incomplete” (red cap):160 mM (NH4)2SO4, 670mM TrisHCl pH8,8, 0,1% Tween-20
Reaction buffer (10X) “complete” (yellow cap):160 mM (NH4)2SO4, 670mM TrisHCl pH8,8, 0,1% Tween-20, 25mM MgCl2
separate Tube: MgCl2 (100 mM, green cap)

Quality control:
Activity and performance test in real time PCR, SDS-PAGE purity, absence of endonucleases/nickases and exonucleases test
 
Usage:  

Use your existing and optimized protocol. In contrast to chemically modified Taq DNA pol. where the first denaturation step needs up to 15 min, Maximo M-Superhot Taq for Real Time PCR does not need a prolonged heating or denaturation time and works excellent basis enzyme for real time PCR.

Components Volume per reaction
 10X reaction buffer  10 µl
 100 mM MgCl2  optional
 dNTP-Mix (40mM)  1.0 µl
 Up-stream primer (10 µM stock)  0,5-2.5 µl
 Down-stream primer (10µM stock)   0.5-2,5 µl
 Template DNA  0.1-15 ng/ml plasmid DNA
 1-10 µg/ml genomic DNA
 Maximo M-Superhot Taq DNA (5 u/µl)   0.2 - 1.0 µl  
 Sterile dest. Water (molecular grade)  up to 50 µl total reaction volume

Note: 
- vortex all solutions carefully before using
- add the enzyme after Template DNA
- may you have to optimize the MgCl2 concentration for best result

General Thermo-Cycler protocol:
 Step  Time  Temperature
 Initial denaturation  2-5 min  94-95°C
 
25-30 Cycles:
 Denaturation
 Annealing
 Extension
 
 10-25 sec
 10-25 sec
 60 sec

 94-95°C
 55-65°C
 72°C per 1kb
 
 Final extension    5 min  72°C

Note: 
- In case of low amount of DNA template, additionally cycles may be used 

Transportation: on blue ice

Storage: at -20°C for 24 months or  for more than 3 months at +4°C


Related products for Hot start PCR:
p-Superhot Taq Polymerase (hot-start PCR)

>>> Service pages in English <<< >>> Serviceseiten auf Deutsch <<<
Copyrightę GeneOn 2007-15 print page: Hot-Start PCR: M-Superhot Taq Polymerase
 


Taq Polymerase
especially as basic enzyme for
RTD-PCR and Hot-Start PCR

m-SUPERHOT TAQ
ultra short in-activation time
 
Start taq polymerase  Hot star Taq Polymerase


related Product:
Ultra pure dNTPs (>99 % HPLC)



 
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Polymerases
 
one-Fusion high-speed-fidelity Polymerase
HighEnd and exclusive Polymerase: faster speed, more accuracy, better result
DFS Taq Polymerase DNA-free Discontinued
DFS Taq Polymerase (DNA free) is a highly purified polymerase (DNA-free) especially for PCR reactions where freedom from endogenous template (i.e., E. coli DNA) is essential
DFS-PLUS Taq Polymerase DNA-free
DFS PlusTaq Polymerase: more sensitivity; more processivity
H-SPlus-Taq DNA Polymerase
Hot Start Polymerase with ultra short inactivation time, free of MAB´s. The high concentrated versions are designed for Lyophilization
Hot-Start PCR: M-Superhot Taq Polymerase
Superior Taq Polymerase for Real Time PCR and Hot-Start PCR, low-copy number PCR, PCR of difficult templates, Hot-Start activity (only 5 min initial denaturation). The enzyme is developed to enhance the specificity, sensitivity and yield of DNA amplification. The enzyme provides a convenient setting up at room temperature
SNPase for SNP-Genotyping
Hot-Start Polymerase for SNP detection by allele-specific PCR and micro sequencing
Taq DNA Polymerase
Maximo Taq Polymerase is a highly purified polymerase for routine amplification
Taq DNA Polymerase 2X-preMix
Maximo Tag Polymerase as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR
Taq DNA Polymerase blue ready-to-load
Maximo Tag-Blue Polymerase is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed!
m-Anti-Taq
Enhancer for Polymerase reactions
p-Taq Polymerase
p-Taq Polymerase is developed to enhance the specificity and yield of DNA amplification. The polymerase provides a convenient setting up at room temperature because of blocking the the enzyme with optimized mix of inhibitors
Bst DNA Polymerase
Bst DNA allows sequencing through problematic secondary structures
Pfu/Psp DNA Polymerase
Pfu DNA polymerase generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragments are blunt-ended for direct ligation
Pfu/Psp red DNA Polymerase RTL
Pfu DNA polymerase (RTU=READY-TO-LOAD) is a convenient Mixture of proof-reading Enzyme, red dye and loading buffer. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Pfu/Psp DNA Polymerase 2X-preMix
Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation
Tth DNA Polymerase
Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium.



 
 
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