| Polymerases |
| Polymerases for Hot Start PCR (qPCR), Standard PCR and proof-reading (high-fidelity) PCR |
| DFS-Taq DNA Polymerase DNA-free |
| Tag DNA Polymerase (DNA free) is a highly purified polymerase (DNA-free) especially for PCR reactions where freedom from endogenous template (i.e., E. coli DNA) is essential |
| Taq DNA Polymerase |
| Maximo Tag DNA Polymerase is a highly purified polymerase for routine amplification |
| Taq DNA Polymerase 2X-preMix |
| Maximo Tag DNA as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR |
| Taq DNA Polymerase blue ready-to-load |
| Maximo Tag-Blue DNA is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed! |
| m-Superhot Taq DNA pol. |
| M-Superhot Taq DNA Polymerase is developed to enhance the specificity, sensitivity and yield of DNA amplification. The enzyme provides a convenient setting up at room temperature |
| p-Superhot Taq DNA pol. |
| p-Superhot Taq DNA Polymerase is developed to enhance the specificity and yield of DNA amplification. The polymerase provides a convenient setting up at room temperature because of blocking the the enzyme with optimized mix of inhibitors |
| Pfu/Psp DNA Polymerase |
| Pfu DNA polymerase generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragments are blunt-ended for direct ligation |
| Pfu/Psp red RTL |
| Pfu DNA polymerase (RTU=READY-TO-LOAD) is a convenient Mixture of proof-reading Enzyme, red dye and loading buffer. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation |
| Pfu/Psp 2X-preMix |
| Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation |
| Tth DNA Polymerase |
| Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium. |
PCR Mastermixes / RTU mixes |
| PCR pre-Mixes from GeneOn are convenient in handling, reduce the risk of contamination and pipetting errors but increase the reproducibility. As ready-to-laod (RTL) version the enzymes helps to reduce working time |
| Taq DNA Polymerase 2X-preMix |
| Maximo Tag DNA as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR |
| Taq-Blue DNA Pol. ready-to-load |
| Maximo Tag-Blue DNA is a highly purified polymerase for routine amplification. The enzyme is ready-to-load. No extra dye or loading buffer is needed! |
| Taq DNA Polymerase 2X-preMix Blue |
| Maximo Tag DNA Blue as 2X-preMix includes all components (Taq DNA polymerase, dNTPs, MgCl2, and reaction buffer) in an optimal concentration for routine PCR. In addition blue dye and loading buffer ensure a direct loading to the agarose gel |
| Pfu/Psp red RTL |
| Pfu DNA polymerase (RTU=READY-TO-LOAD) is a convenient Mixture of proof-reading Enzyme, red dye and loading buffer. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation |
| Pfu/Psp 2X-preMix |
| Pfu DNA polymerase 2X-preMix is a convenient Mixture of proof-reading Enzyme, reaction buffer dNTP´s and MgCl2. It generated PCR fragments exhibits the lowest error rate of any thermostable DNA polymerases. The fragements are blunt-ended for direct ligation |
Nucleotides |
| dNTP´s from GeneOn are available in sets of 4 single dNTPs or as 10 mM ready-to-use mixes |
| dNTP Set / pre-Mixture |
| dNTP´s from GeneOn are available in sets of 4 single dNTPs or as 10 mM ready-to-use mixes |
Reverse Transcription |
| Reverase transcription with AMV or MMLV can uses either RNA or DNA to prime DNA synthesis |
| MMLV Reverse Transcription |
| MMLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (MMLV RT) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized |
| AMV Reverse Transcription |
| AMV Reverse Transcriptase, encoded by Avian Myeloblastosis Virus reverse transcriptase and expressed in E.coli. is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template |
| Ribunuclease Inhibitor |
| RNase Inhibitor is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C |
| Tth DNA Polymerase |
| Tth DNA Polymerase Maximo is used to reverse-transcribe RNA efficiently in the presence of manganese. PCR can be performed in the same tube using the intrinsic DNA polymerase activity simply by chelation of manganese cation and the addition of magnesium. |
Enzymes/chemicals for PCR |
| Assorted products for PCR and finechemicals for Molecular Biology |
| Uracil-DNA Glycosylase |
| Uracil-DNA Glycosylase (UDG) prevents carry over of DNA in PCR reactions |
| T 4 DNA Ligase |
| T 4 DNA Ligase high concentrated catalyzes the formation of a phosphodiester bond between juxtaposed 5´ phosphate and 3´ hydroxyl termini in duplex DNA or RNA. |
| Agarose 50bp-50kbp |
| Universal Agarose from GeneON is ideal for use as standard agarose for analytical as well as preparative nucleic acid electrophoresis of fragments from 50 bp to 50 kbp. Even at low concentrations the gel produced is very firm |
| Proteinase K |
| Proteinase K isolated from Tritirachium album is used for protease digestion during DNA and RNA preparation. Proteinase K from GeneON is very active and stable |
| IPTG |
| IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening |
| X-Gal |
| X-Gal: On combination with suitable bacterial cloning vectors, host strains, |
Light Cycler Capillaries |
| Light Cycler Capillaries from optical Polycarbonate is a clever alternative to the Glas-capillary |
