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Sibenzyme - Restriction
Endonucleases
Alu I
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SmaI
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SphI
SseBI
SspI
SstI
StyI
TaqI
XbaI
Dialysis Membranes
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You are here : Sibenzyme - Restriction Endonucleases ╗ EcoRV

EcoRV

Sequence of EcoRV: 5┤...GATATC...3┤ 3┤...CTATAG...5┤

 
 

Discontinued product – For substitute please visit:

Restriction endonucleases from Sibenzyme



Catalog-no

Description

units

Euro

Order by E-mail

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250-115S  EcoR V 3000 u 

18.00

EcoR V  3000 u Add

250-115L

 EcoR V  5x3000 u 90.00 EcoR V  15000 u Add


Overhang: 5´ - blunt


Cut Site:

G A T / A T C
C T A / T A G


Isoschizomers: Eco32 I

Neoschizomers: N/A

Source: Escherichia coli, J62plg 74.

Buffer supplied:
10x M

Substrate for unit definition:
  λ DNA

Reaction conditions:
50 mM NaCl, 10 mM Tris-HCl (pH 7.9 at 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37°C

Storage buffer:
50 mM KCl, 10 mM Tris-HCl  (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol

Store: at -20°C.

Absence of contaminants:
Fifty units of EcoRV do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C.  After 20-fold overdigestion with EcoRV, greater than 95% of the DNA fragments can be ligated and recut with this enzyme
 
Heat inactivation: 80°C for 20 minutes

Star activity:
Conditions of low ionic strength, high enzyme concentration, glycerol concentration>5%, or pH>8.0 may result in star activity

Note: Activity may be blocked by overlapping CpG methylation.


Table of Restriction Endonucleases

Alu I - BseC I BshF I - Hpa I Kpn I - Pvu II Rsa I - SnaB I SseB I - Xba I
Alu I BshF I Kpn I Rsa I  SseB I
ApaL I BsiS I (N/A) Mbo I  Sal I Sst I
Asu II BssA I MspC I Sau3A I  Sty I
BamH I BstE II Nae I Sca I Taq I
Bcl I CspA I Nco I Sfi I  Xba I
Bgl I EcoR I Nhe I SgrB I (Sac II)  
Bgl II EcoR V Not I Sla I  
BseA I Hind III Nru I Sma I  
BseB I Hinf I Pst I SnaB I  
BseC I Hpa I Pvu II Sph I  


Table of Isoschizomeres

Isosch. Link to
Enzmye
Isosch. Link to
Enzmye
Isosch. Link to
Enzmye
Isosch. Link to
Enzmye
Aat I SseB I BsiX  BseC I Cbi I  Asu II  NgoM IV  Nae I 
Acc III BseA I BIsp106 I  BseC I  Cfr42 I  SgrB I  NspV  Asu II
Afi II MspC I Bsp119 I  Asu II  Cla I  BseC I  PaeR7 I  Sla I
Age I CspA I Bsp13 I  BseA I  Csp45 I Asu II  Pal I  BshF I
AsiA II CspA I BspD I  BseC I  Eco147 I  SseB I  PinA I  CspA I
Ban III BseC I BspE I  BseA I  EcoR II  BseB I  Pm55 II  SseB I
Bfr I MspCI BspT 1  MspC I  Hae III  BshF I  PspG I  BseB I
Bpu14 I Asu II BspX I  BseC I  Kpn I  BseA I  Sac II  SgrB I
Bsa29 I BseC I  Bst2U I  BseB I  Ksp I  SgrB I  Sfr274 I  Sla I
Bsc I BseC I  Bst98 I  MspC I  Lsp I  Asu II  Sfr303 I  SgrB I
Bsh I BshF I  BstB I  Asu II  Mro I  BseA I  Sfu I  Asu II
Bsh T I CspA I  BstN I  BseB I  MroN I  Nae I  Sst II  SgrB I
BsiC I Asu II  BstO I  BseB I  Msp I  BsiS I  Stu I  SseB I
BsiL I BseB I  Bsu15 I  BseC I  Mva I  BseB I  Tlil  Sla I
BsiM I BseA I  BsuR I  BshF I  NgoA IV  Nae I  Xho I  SseB I

 


 

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Copyrightę GeneOn 2007-14 print page: EcoRV
 


 
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