EcoRI

Sequence of EcoRI: 5´...GAATTC...3´ 3´...CTTAAG...5´

Catalog-no	Description	units	Euro	Order by E-mail	Shop
250-114S	EcoRI	5000 u	10.00	EcoRI 5000 u	Add
250-114L	EcoRI	5x5000 u	40.00	EcoRI 25000 u	Add

EcoRI: Datsheet
EcoRI: Order by E-Mail

Overhang: 5´ - AATT

Cut Site:

G / A A T T C
C T T A A / G

Isoschizomers: FunII

Neoschizomers: N/A

Source: E. coli RY 13.

Buffer supplied: 10x EcoR I.

Substrate for unit definition: λ DNA.

Reaction conditions: 50 mM NaCl, 100 mM Tris-HCl (pH 7.9 at 25°C), 5 mM MgCl₂, 0.025% Triton X-100, 100 μg/ml BSA. Incubate at 37°C.

Storage buffer: 300 mM NaCl, 5 mM KPO4 (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg/ml BSA and 50% glycerol.

Store: at -20°C.

Absence of contaminants: Ten units of EcoRI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After 50-fold overdigestion with EcoRI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

Heat inactivation: 65°C for 20 minutes.

Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration>5%, or pH>8.0 may result in star activity.

Note: Activity may be impaired by overlapping CpG methylation.

Table of Restriction Endonucleases

Alu I - BseC I	BshF I - Hpa I	Kpn I - Pvu II	Rsa I - SnaB I	SseB I - Xba I
Alu I	BshF I	Kpn I	Rsa I	SseB I
ApaL I	BsiS I (N/A)	Mbo I	Sal I	Sst I
Asu II	BssA I	MspC I	Sau3A I	Sty I
BamH I	BstE II	Nae I	Sca I	Taq I
Bcl I	CspA I	Nco I	Sfi I	Xba I
Bgl I	EcoR I	Nhe I	SgrB I (Sac II)
Bgl II	EcoR V	Not I	Sla I
BseA I	Hind III	Nru I	Sma I
BseB I	Hinf I	Pst I	SnaB I
BseC I	Hpa I	Pvu II	Sph I

Table of Isoschizomeres

Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye Isosch. Link to
Enzmye

Aat I SseB I BsiX BseC I Cbi I Asu II NgoM IV Nae I

Acc III BseA I BIsp106 I BseC I Cfr42 I SgrB I NspV Asu II

Afi II MspC I Bsp119 I Asu II Cla I BseC I PaeR7 I Sla I

Age I CspA I Bsp13 I BseA I Csp45 I Asu II Pal I BshF I

AsiA II CspA I BspD I BseC I Eco147 I SseB I PinA I CspA I

Ban III BseC I BspE I BseA I EcoR II BseB I Pm55 II SseB I

Bfr I MspCI BspT 1 MspC I Hae III BshF I PspG I BseB I

Bpu14 I Asu II BspX I BseC I Kpn I BseA I Sac II SgrB I

Bsa29 I BseC I Bst2U I BseB I Ksp I SgrB I Sfr274 I Sla I

Bsc I BseC I Bst98 I MspC I Lsp I Asu II Sfr303 I SgrB I

Bsh I BshF I BstB I Asu II Mro I BseA I Sfu I Asu II

Bsh T I CspA I BstN I BseB I MroN I Nae I Sst II SgrB I

BsiC I Asu II BstO I BseB I Msp I BsiS I Stu I SseB I

BsiL I BseB I Bsu15 I BseC I Mva I BseB I Tlil Sla I

BsiM I BseA I BsuR I BshF I NgoA IV Nae I Xho I SseB I

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