Cat.-no	Description	Amount	Price €	Shop
N105	Maximo DFS-Taq Polymerase	500 units	60.00	add
N106	Maximo DFS-Taq Polymerase	5x500 units	339.00	add
N107	Maximo DFS-Taq Polymerase	20x500 units	899.00	add
N108	Maximo DFS-Taq Polymerase	100x500 units   or as bulk	2500.00	add
N108X	Maximo DFS-Taq Polymerase	other amounts  other conc.	on request

The standard concentration of Maximo DFS-Taq is 5 u/µl. On request we can supplier concentrations up to 50 u/µl.

Maximo Taq Polymerase ^DNA-free:Order/request by E-mail:
Maximo Taq Polymerase ^DNA-free: Datasheet
Maximo Taq Polymerase ^DNA-free: Tests and Evaluation sheet
Maximo Taq Polymerase ^DNA-free: Deutsche Beschreibung


Features:
Researchers are often encounting with contaminating DNA present in their polymerase preparations that often preclude or obscure accurate interpretation of PCR results, especially when targeting conserved sequences. Maximo DFS-Taq Polymerase is ideal in detecting and identifying bacterial DNA, looking for a more accurate method in mutation scanning techniques, or wanting to prevent the amplification of undesired DNA sequences.

Applications Taq Polymerase:
- Standard / General PCR
- PCR with bacterial DNA
- Quantitative PCR
- E. coli contamination studies
- Microbial (i.e., 16S/23S) contamination
  studies
- Forensic studies
- PCR cloning
- RT-PCR 

Description: 
Maximo Taq Polymerase is a recombinant thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a double-strand specific 5´→3´ exonuclease activity. The enzyme consists of a single polypeptide with a molecular weight of 94KD. The enzyme is free of DNA-contaminations.

Concentration:  5 u/µl

Unit definition:
One unit incorporates 10 nmol of deoxyribonucleotide into acid-precipitation material in 30min at 74 degree

Storage Buffer:
25 mM Tris-HCl (pH 8.0), 150 mM NaCl, 0.05 mM EDTA, 1 mM DTT, 50% glycerol.

Reaction Buffers supplied with Taq Polymerase:

10X Buffer I:
200mM Tris-HCl, 100 mM KCl, 100mM (NH₄)₂S0₄, 1% Triton X-100, at pH 8.8 (25°C)
10X Buffer II: 
200mM Tris-HCl, 100 mM KCl, 20mM MgSO₄, 100mM (NH₄)₂S0₄, 1% Triton X-100, at pH 8.8 (25°C)
MgCl₂: 25 mM




Quality control: 
- E. coli genomic DNA testing after
  treatment  with T5 DNAse
- PCR with various templates – human and 
  bovine
  genomic DNA, Phage Lambda DNA
- 3 kb DNA amplification from 50 ng DNA
- batch variation 
 
Transportation: on blue ice

Storage: at -20°C for 12 months




Usage:  

Components	Volume per reaction
10X reaction buffer I	5 µl
25 mM MgCl2	1.5 µl (if necessary)
dNTP-Mix (40mM)	1.0 µl
Up-stream primer (10 µM stock)	0,5-2.5 µl
Down-stream primer (10µM stock)	0.5-2,5 µl
Template DNA	0.1-15 ng/ml plasmid DNA  1-10 µg/ml genomic DNA
Maximo Taq DNA Polymerase (5 u/µl)	0.2 - 1.0 µl
Sterile dest. Water (molecular grade)	up to 50 µl total reaction volume

Note: 
- vortex all solutions carefully before using
- dispense all reagents on ice
- add the enzyme after Template DNA
- may you have to optimize the MgCl₂ concentration for best result

General Thermo-Cycler protocol:

Step	Time	Temperature
Initial denaturation	2-5 min	94-95°C
25-30 Cycles:  Denaturation  Annealing  Extension	10-25 sec  10-25 sec  60 sec	94-95°C  55-65°C  72°C per 1kb
Final extension	5 min	72°C

Related products to DFS Taq DNA Polymerase:

dNTP's mixture

dNTP's set

MMLV Reverase Transcriptase

AMV Reverase Transcriptase

T 4 DNA Ligase

50 bp DNA Ladder

M-Superhot Taq Polymerase

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