Cloning Vectors / Phage DNA

Phage and Cloning Vectors are used as a substrate in restriction enzyme activity assays and for preparation of DNA molecular weight standards

Cloning Vectors / Phagen DNA	Description
Phage T7 DNA	Infected E. coli strain of linear dsDNA, 39937 bp in length
pBR322	E.coli cloning vector, 4361 bp in length
pUC18 DNA	E.coli vector, 2686 base pairs in length
Phage λ-DNA	E.coli W3110 (cI857 Sam7) strain of linear dsDNA, 48502 base pairs in length

Overview this section:

pBR322 cloning vector

pBR322 is a commonly used plasmid cloning vector in E. coli

pUC18/19 is a commonly used plasmid cloning vector in E. coli.

Phage T7 DNA is an alternative to Lambda DNA for the restriction endonucleases

Phage Lambda DNA is used in restriction enzymes research and for testing of restriction endonucleases activity.