Bovine Serum Albumin - BSA
BSA prevent the adhesion of enzymes at reaction tubes
| Cat.-no | Description | Amount | Price € | Shop |
| 209-001 | Bovine Serum Albumin (BSA) | 1,5 ml | 7.00 | add |
| 209-005 | Bovine Serum Albumin (BSA) | 4x1,5 ml | 25.00 | add |
BSA (Bovine Serum Albumin) Datasheet:
BSA (Bovine Serum Albumin) Deutsche Beschreibung:
Description:
Bovine Serum Albumin (BSA) is supplied with some restriction enzymes to prevent adhesion of the enzyme to reaction tubes and pipette surfaces. BSA also stabilizes some proteins during incubation. The source of purified BSA is a molecular biology grade, protease free, fraction V starting material. This material is then processed to remove nucleases.
Concentration: 10 mg/ml
Storage Conditions:
20 mM KPO4
50 mM NaCl
0.1 mM EDTA
5% Glycerol
pH 7.0 (25°C)
Storage Temperature: -20°C
Quality Control:
A 16 hour incubation is performed with ΦX174 DNA- HaeIII Digest. This assay checks for endonuclease, exonuclease and binding protein contamination. 16 hour incubation is performed with ΦX174 RFI DNA, as sensitive assay for nonspecific endonuclease and 1 hour incubation with a RNA ladder is performed
Transport: Ambient or with blue ice
Note:
- It is do not recommend using this preparation of BSA as a standard for quantitation purposes or size
determination.
- The BSA should be diluted 1:100 for standard reactions. At this level the buffer from the BSA have a very small effect, only.
Related products:
Restriction Endonucleases
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